Detailed information of TA system TA022487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 97242..97885
• Replicon: plasmid pAPEC-O1-ColBM
• Accession: NC_009837
• Organism: Escherichia coli APEC O1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: APECO1_RS26235
• Protein ID: WP_001044768.1
• Coordinates: 97242..97658 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: APECO1_RS26240
• Protein ID: WP_001261287.1
• Coordinates: 97655..97885 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO1_RS26205	92346..92630	+	285	WP_012006537.1	ribbon-helix-helix domain-containing protein	-
APECO1_RS26210	92630..92905	+	276	WP_000421266.1	type II toxin-antitoxin system RelE/ParE family toxin	-
APECO1_RS26215	93011..93250	+	240	WP_001105068.1	hypothetical protein	-
APECO1_RS26230	94942..97080	+	2139	WP_000350635.1	AAA family ATPase	-
APECO1_RS26235	97242..97658	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
APECO1_RS26240	97655..97885	-	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
APECO1_RS26245	98447..98797	+	351	WP_000493379.1	hypothetical protein	-
APECO1_RS26250	98841..99530	+	690	WP_000796229.1	hypothetical protein	-
APECO1_RS26255	99527..100318	+	792	WP_000016494.1	site-specific integrase	-
APECO1_RS26260	100496..100849	+	354	WP_000864812.1	colicin M immunity protein	-
APECO1_RS26265	100899..101714	-	816	WP_001312845.1	lipid II-degrading bacteriocin colicin M	-
APECO1_RS26270	101958..102485	+	528	WP_000203272.1	colicin B immunity protein	-
APECO1_RS29825	102503..102670	-	168	Protein_112	colicin-B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..174241	174241
	-	inside	IScluster/Tn	-	-	78096..94240	16144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T22487 WP_001044768.1 NC_009837:c97658-97242 [Escherichia coli APEC O1]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T22487 NC_009837:c97658-97242 [Escherichia coli APEC O1]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT22487 WP_001261287.1 NC_009837:c97885-97655 [Escherichia coli APEC O1]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT22487 NC_009837:c97885-97655 [Escherichia coli APEC O1]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4