Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 482364..483001 Replicon chromosome
Accession NZ_CP087137
Organism Bacillus velezensis strain JIN4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LOK79_RS02485 Protein ID WP_003156187.1
Coordinates 482651..483001 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag LOK79_RS02480 Protein ID WP_003156188.1
Coordinates 482364..482645 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOK79_RS02460 (LOK79_02460) 478730..479329 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
LOK79_RS02465 (LOK79_02465) 479422..479787 + 366 WP_014304402.1 holo-ACP synthase -
LOK79_RS02470 (LOK79_02470) 479951..480958 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
LOK79_RS02475 (LOK79_02475) 481075..482244 + 1170 WP_058906584.1 alanine racemase -
LOK79_RS02480 (LOK79_02480) 482364..482645 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LOK79_RS02485 (LOK79_02485) 482651..483001 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LOK79_RS02490 (LOK79_02490) 483119..483940 + 822 WP_014304404.1 STAS domain-containing protein -
LOK79_RS02495 (LOK79_02495) 483945..484310 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
LOK79_RS02500 (LOK79_02500) 484313..484714 + 402 WP_003156178.1 anti-sigma regulatory factor -
LOK79_RS02505 (LOK79_02505) 484726..485733 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
LOK79_RS02510 (LOK79_02510) 485797..486126 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
LOK79_RS02515 (LOK79_02515) 486123..486605 + 483 WP_003156173.1 anti-sigma B factor RsbW -
LOK79_RS02520 (LOK79_02520) 486571..487359 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
LOK79_RS02525 (LOK79_02525) 487359..487961 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T224858 WP_003156187.1 NZ_CP087137:482651-483001 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T224858 NZ_CP120567:2459598-2459700 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT224858 WP_003156188.1 NZ_CP087137:482364-482645 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT224858 NZ_CP120567:c2459738-2459470 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References