Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 68975..69618 Replicon plasmid pAPEC-O1-ColBM
Accession NC_009837
Organism Escherichia coli APEC O1

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag APECO1_RS26085 Protein ID WP_001034044.1
Coordinates 68975..69391 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag APECO1_RS26090 Protein ID WP_001261286.1
Coordinates 69388..69618 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO1_RS26070 64534..66075 - 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
APECO1_RS26075 66313..67350 - 1038 WP_000361403.1 hypothetical protein -
APECO1_RS26080 67335..68900 - 1566 WP_001128474.1 AAA family ATPase -
APECO1_RS26085 68975..69391 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
APECO1_RS26090 69388..69618 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
APECO1_RS26095 69980..70432 + 453 WP_000670960.1 N-acetyltransferase -
APECO1_RS26100 70464..71996 - 1533 WP_001017349.1 NAD(P)-binding domain-containing protein -
APECO1_RS26105 71993..72262 - 270 WP_000379710.1 SemiSWEET transporter -
APECO1_RS26110 72264..73280 - 1017 WP_000583428.1 Gfo/Idh/MocA family oxidoreductase -
APECO1_RS26115 73283..74110 - 828 WP_000154003.1 HAD-IIB family hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..174241 174241


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T22485 WP_001034044.1 NC_009837:c69391-68975 [Escherichia coli APEC O1]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T22485 NC_009837:c69391-68975 [Escherichia coli APEC O1]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT22485 WP_001261286.1 NC_009837:c69618-69388 [Escherichia coli APEC O1]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT22485 NC_009837:c69618-69388 [Escherichia coli APEC O1]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References