Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3034322..3034976 Replicon chromosome
Accession NZ_CP087136
Organism Escherichia coli strain JW5503

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LOX89_RS14840 Protein ID WP_000244777.1
Coordinates 3034322..3034729 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LOX89_RS14845 Protein ID WP_000354046.1
Coordinates 3034710..3034976 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX89_RS14820 (3030279) 3030279..3032012 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
LOX89_RS14825 (3032018) 3032018..3032728 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LOX89_RS14830 (3032753) 3032753..3033649 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LOX89_RS14835 (3033761) 3033761..3034282 + 522 WP_001055874.1 flavodoxin FldB -
LOX89_RS14840 (3034322) 3034322..3034729 - 408 WP_000244777.1 protein YgfX Toxin
LOX89_RS14845 (3034710) 3034710..3034976 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LOX89_RS14850 (3035219) 3035219..3036199 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LOX89_RS14855 (3036395) 3036395..3037054 - 660 WP_000250274.1 hemolysin III family protein -
LOX89_RS14860 (3037218) 3037218..3037529 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LOX89_RS14865 (3037574) 3037574..3039007 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LOX89_RS14870 (3039064) 3039064..3039807 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T224845 WP_000244777.1 NZ_CP087136:c3034729-3034322 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224845 NZ_CP120564:c44444-44169 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT224845 WP_000354046.1 NZ_CP087136:c3034976-3034710 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224845 NZ_CP120564:c44722-44444 [Escherichia coli]
ATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGACCAGAT
AGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGGAGCGTA
ATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGGGCGGAC
AGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References