Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2771021..2771688 Replicon chromosome
Accession NZ_CP087136
Organism Escherichia coli strain JW5503

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag LOX89_RS13625 Protein ID WP_001094400.1
Coordinates 2771359..2771688 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag LOX89_RS13620 Protein ID WP_000072690.1
Coordinates 2771021..2771338 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX89_RS13590 (2766073) 2766073..2767082 - 1010 Protein_2667 arsenic transporter -
LOX89_RS13595 (2767224) 2767224..2768927 + 1704 WP_000896263.1 protein YfjW -
LOX89_RS13600 (2769499) 2769499..2769722 + 224 Protein_2669 DUF905 family protein -
LOX89_RS13605 (2769825) 2769825..2770283 + 459 WP_000211841.1 antirestriction protein -
LOX89_RS13610 (2770292) 2770292..2770774 + 483 WP_001407480.1 RadC family protein -
LOX89_RS13615 (2770783) 2770783..2770983 + 201 WP_001395452.1 DUF987 domain-containing protein -
LOX89_RS13620 (2771021) 2771021..2771338 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LOX89_RS13625 (2771359) 2771359..2771688 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
LOX89_RS13630 (2772052) 2772052..2776632 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T224843 WP_001094400.1 NZ_CP087136:2771359-2771688 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T224843 NZ_CP120563:c4512552-4512379 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT224843 WP_000072690.1 NZ_CP087136:2771021-2771338 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT224843 NZ_CP120563:c4512876-4512580 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References