Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2071140..2071971 Replicon chromosome
Accession NZ_CP087136
Organism Escherichia coli strain JW5503

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag LOX89_RS10330 Protein ID WP_000854814.1
Coordinates 2071597..2071971 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag LOX89_RS10325 Protein ID WP_001285584.1
Coordinates 2071140..2071508 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX89_RS10310 (2068807) 2068807..2070339 + 1533 WP_001350525.1 protein YeeR -
LOX89_RS10315 (2070336) 2070336..2070782 + 447 WP_000187523.1 RadC family protein -
LOX89_RS10320 (2070845) 2070845..2071066 + 222 WP_000692323.1 DUF987 domain-containing protein -
LOX89_RS10325 (2071140) 2071140..2071508 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LOX89_RS10330 (2071597) 2071597..2071971 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
LOX89_RS10335 (2071968) 2071968..2072162 + 195 WP_000988600.1 DUF5983 family protein -
LOX89_RS10340 (2072208) 2072208..2072288 + 81 Protein_2027 hypothetical protein -
LOX89_RS10345 (2072577) 2072577..2072705 - 129 Protein_2028 transposase domain-containing protein -
LOX89_RS10350 (2072825) 2072825..2072959 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
LOX89_RS10355 (2073060) 2073060..2073389 - 330 WP_000450409.1 DUF496 family protein -
LOX89_RS10360 (2073561) 2073561..2074619 - 1059 WP_001200891.1 FUSC family protein -
LOX89_RS10365 (2074817) 2074817..2075290 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
LOX89_RS10370 (2075409) 2075409..2076575 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T224840 WP_000854814.1 NZ_CP087136:2071597-2071971 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T224840 NZ_CP120563:3588086-3588304 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT224840 WP_001285584.1 NZ_CP087136:2071140-2071508 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT224840 NZ_CP120563:3587686-3588060 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References