Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 56489..57024 Replicon plasmid pAPEC-O1-ColBM
Accession NC_009837
Organism Escherichia coli APEC O1

Toxin (Protein)


Gene name relE Uniprot ID A0A0H2XJT3
Locus tag APECO1_RS26035 Protein ID WP_000222767.1
Coordinates 56737..57024 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag APECO1_RS26030 Protein ID WP_001132900.1
Coordinates 56489..56740 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO1_RS25995 51616..52176 + 561 WP_000139315.1 fertility inhibition protein FinO -
APECO1_RS26000 52312..52524 + 213 WP_001299730.1 ANR family transcriptional regulator -
APECO1_RS31665 52770..52844 + 75 Protein_61 endonuclease -
- 53100..53161 - 62 NuclAT_1 - -
- 53100..53161 - 62 NuclAT_1 - -
- 53100..53161 - 62 NuclAT_1 - -
- 53100..53161 - 62 NuclAT_1 - -
APECO1_RS26005 53148..53354 + 207 WP_001351576.1 type I toxin-antitoxin system Hok family toxin -
APECO1_RS26010 53638..53895 + 258 WP_000083845.1 replication regulatory protein RepA -
APECO1_RS26015 54127..54201 + 75 WP_001442103.1 RepA leader peptide Tap -
APECO1_RS26020 54194..54676 + 483 WP_001273588.1 hypothetical protein -
APECO1_RS26025 54669..55526 + 858 WP_000774297.1 incFII family plasmid replication initiator RepA -
APECO1_RS26030 56489..56740 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
APECO1_RS26035 56737..57024 + 288 WP_000222767.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..174241 174241


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11190.26 Da        Isoelectric Point: 10.6914

>T22484 WP_000222767.1 NC_009837:56737-57024 [Escherichia coli APEC O1]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKRRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T22484 NC_009837:56737-57024 [Escherichia coli APEC O1]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACGAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT22484 WP_001132900.1 NC_009837:56489-56740 [Escherichia coli APEC O1]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT22484 NC_009837:56489-56740 [Escherichia coli APEC O1]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2XJT3

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References