Detailed information of TA system TA224829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1504090..1504728
• Replicon: chromosome
• Accession: NZ_CP087136
• Organism: Escherichia coli strain JW5503

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LOX89_RS07440
• Protein ID: WP_000813794.1
• Coordinates: 1504090..1504266 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LOX89_RS07445
• Protein ID: WP_001270286.1
• Coordinates: 1504312..1504728 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX89_RS07420 (1499709)	1499709..1500923	-	1215	WP_001326689.1	BenE family transporter YdcO	-
LOX89_RS07425 (1500976)	1500976..1501512	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LOX89_RS07430 (1501585)	1501585..1503546	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LOX89_RS07435 (1503638)	1503638..1503868	-	231	WP_000494244.1	YncJ family protein	-
LOX89_RS07440 (1504090)	1504090..1504266	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LOX89_RS07445 (1504312)	1504312..1504728	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LOX89_RS07450 (1504807)	1504807..1506213	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LOX89_RS07455 (1506458)	1506458..1507603	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LOX89_RS07460 (1507621)	1507621..1508634	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LOX89_RS07465 (1508635)	1508635..1509576	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T224829 WP_000813794.1 NZ_CP087136:1504090-1504266 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T224829 NZ_CP120563:c605760-605296 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT224829 WP_001270286.1 NZ_CP087136:1504312-1504728 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT224829 NZ_CP120563:c606095-605760 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 6HPB