Detailed information of TA system TA224817

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 259039..259718
• Replicon: chromosome
• Accession: NZ_CP087136
• Organism: Escherichia coli strain JW5503

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: LOX89_RS01235
• Protein ID: WP_000854672.1
• Coordinates: 259039..259380 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: LOX89_RS01240
• Protein ID: WP_000070395.1
• Coordinates: 259401..259718 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX89_RS01210 (254316)	254316..254717	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
LOX89_RS01215 (254756)	254756..255811	-	1056	WP_000749863.1	phosphoporin PhoE	-
LOX89_RS01220 (256099)	256099..257202	+	1104	WP_001285288.1	glutamate 5-kinase	-
LOX89_RS01225 (257214)	257214..258467	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
LOX89_RS01235 (259039)	259039..259380	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
LOX89_RS01240 (259401)	259401..259718	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
LOX89_RS01245 (259737)	259737..259958	-	222	WP_000691994.1	DUF987 domain-containing protein	-
LOX89_RS01250 (259967)	259967..260443	-	477	WP_000811693.1	RadC family protein	-
LOX89_RS01255 (260459)	260459..260917	-	459	WP_000211838.1	antirestriction protein	-
LOX89_RS01260 (261015)	261015..261254	-	240	WP_000194654.1	DUF905 family protein	-
LOX89_RS01265 (261331)	261331..261798	-	468	WP_001547765.1	protein YkfB	-
LOX89_RS01270 (261821)	261821..262264	-	444	WP_000824223.1	lipoprotein YafY	-
LOX89_RS01275 (262264)	262264..262491	-	228	WP_001548158.1	protein YpjK	-
LOX89_RS01280 (262487)	262487..262678	-	192	Protein_248	DeoR family transcriptional regulator	-
LOX89_RS01285 (262895)	262895..263716	-	822	WP_000197389.1	DUF932 domain-containing protein	-
LOX89_RS01290 (263808)	263808..264671	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T224817 WP_000854672.1 NZ_CP087136:c259380-259039 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T224817 NZ_CP120557:c4411226-4410948 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT224817 WP_000070395.1 NZ_CP087136:c259718-259401 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT224817 NZ_CP120557:c4411489-4411229 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684