Detailed information of TA system TA224808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5472872..5473542
• Replicon: chromosome
• Accession: NZ_CP087123
• Organism: Klebsiella pneumoniae strain TH12908

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LOX65_RS26360
• Protein ID: WP_004213072.1
• Coordinates: 5473099..5473542 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LOX65_RS26355
• Protein ID: WP_004213073.1
• Coordinates: 5472872..5473102 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX65_RS26330 (5469095)	5469095..5469994	-	900	WP_004225022.1	nucleotide-binding protein	-
LOX65_RS26335 (5469984)	5469984..5470274	-	291	WP_004213078.1	hypothetical protein	-
LOX65_RS26340 (5470626)	5470626..5470832	+	207	WP_004213077.1	hypothetical protein	-
LOX65_RS26345 (5470822)	5470822..5471115	-	294	WP_004213076.1	hypothetical protein	-
LOX65_RS26350 (5471131)	5471131..5472264	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LOX65_RS26355 (5472872)	5472872..5473102	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LOX65_RS26360 (5473099)	5473099..5473542	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOX65_RS26365 (5473691)	5473691..5473942	+	252	WP_186987481.1	hypothetical protein	-
LOX65_RS26370 (5473965)	5473965..5474269	-	305	Protein_5155	transposase	-
LOX65_RS26375 (5474686)	5474686..5475323	+	638	Protein_5156	mucoid phenotype regulator RmpA2	-
LOX65_RS26380 (5475841)	5475841..5476244	-	404	Protein_5157	GAF domain-containing protein	-
LOX65_RS26385 (5476335)	5476335..5477255	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
LOX65_RS26390 (5477304)	5477304..5477795	-	492	WP_011251327.1	DM13 domain-containing protein	-
LOX65_RS26395 (5477858)	5477858..5478124	-	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	5414866..5612194	197328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T224808 WP_004213072.1 NZ_CP087123:5473099-5473542 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T224808 NZ_CP120557:c1414722-1414468 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT224808 WP_004213073.1 NZ_CP087123:5472872-5473102 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT224808 NZ_CP120557:c1414970-1414719 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620