Detailed information of TA system TA224783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5436103..5436746
• Replicon: chromosome
• Accession: NZ_CP087122
• Organism: Klebsiella pneumoniae strain TH12852

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LOX64_RS26475
• Protein ID: WP_114473036.1
• Coordinates: 5436330..5436746 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LOX64_RS26470
• Protein ID: WP_001261276.1
• Coordinates: 5436103..5436333 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX64_RS26430 (5431236)	5431236..5431349	+	114	WP_014343462.1	hypothetical protein	-
LOX64_RS26435 (5431478)	5431478..5431735	+	258	WP_114473033.1	hypothetical protein	-
LOX64_RS26440 (5431793)	5431793..5432572	-	780	WP_064182282.1	site-specific integrase	-
LOX64_RS26445 (5432770)	5432770..5433786	-	1017	WP_114473034.1	hypothetical protein	-
LOX64_RS26450 (5433783)	5433783..5434106	-	324	WP_070552900.1	hypothetical protein	-
LOX64_RS26455 (5434133)	5434133..5434534	-	402	WP_074194759.1	hypothetical protein	-
LOX64_RS26460 (5434571)	5434571..5435515	-	945	WP_064182279.1	hypothetical protein	-
LOX64_RS26465 (5435676)	5435676..5436050	-	375	WP_114473035.1	hypothetical protein	-
LOX64_RS26470 (5436103)	5436103..5436333	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LOX64_RS26475 (5436330)	5436330..5436746	+	417	WP_114473036.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOX64_RS26480 (5436787)	5436787..5437665	-	879	WP_064182004.1	restriction endonuclease	-
LOX64_RS26485 (5438326)	5438326..5439603	+	1278	WP_114473037.1	HlyD family secretion protein	-
LOX64_RS26490 (5439593)	5439593..5441701	+	2109	WP_114473038.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15120.58 Da
• Isoelectric Point: 8.5464

>T224783 WP_114473036.1 NZ_CP087122:5436330-5436746 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILHWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T224783 NZ_CP120549:2253686-2253788 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT224783 WP_001261276.1 NZ_CP087122:5436103-5436333 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT224783 NZ_CP120549:c2253826-2253558 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A387K023