Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5124718..5125343 Replicon chromosome
Accession NZ_CP087122
Organism Klebsiella pneumoniae strain TH12852

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LOX64_RS24815 Protein ID WP_002882817.1
Coordinates 5124718..5125101 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LOX64_RS24820 Protein ID WP_004150355.1
Coordinates 5125101..5125343 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX64_RS24800 (5122084) 5122084..5122986 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LOX64_RS24805 (5122983) 5122983..5123618 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LOX64_RS24810 (5123615) 5123615..5124544 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LOX64_RS24815 (5124718) 5124718..5125101 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LOX64_RS24820 (5125101) 5125101..5125343 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LOX64_RS24825 (5125548) 5125548..5126465 + 918 WP_229982395.1 alpha/beta hydrolase -
LOX64_RS24830 (5126479) 5126479..5127420 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LOX64_RS24835 (5127465) 5127465..5127902 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LOX64_RS24840 (5127899) 5127899..5128759 - 861 WP_004146232.1 virulence factor BrkB family protein -
LOX64_RS24845 (5128753) 5128753..5129352 - 600 WP_071068301.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T224781 WP_002882817.1 NZ_CP087122:c5125101-5124718 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T224781 NZ_CP120549:1948046-1948300 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT224781 WP_004150355.1 NZ_CP087122:c5125343-5125101 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT224781 NZ_CP120549:1947798-1948049 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References