Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3911943..3912562 Replicon chromosome
Accession NZ_CP087122
Organism Klebsiella pneumoniae strain TH12852

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LOX64_RS19095 Protein ID WP_002892050.1
Coordinates 3912344..3912562 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LOX64_RS19090 Protein ID WP_002892066.1
Coordinates 3911943..3912317 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX64_RS19080 (3907095) 3907095..3908288 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LOX64_RS19085 (3908311) 3908311..3911457 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LOX64_RS19090 (3911943) 3911943..3912317 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LOX64_RS19095 (3912344) 3912344..3912562 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LOX64_RS19100 (3912721) 3912721..3913287 + 567 WP_002892030.1 maltose O-acetyltransferase -
LOX64_RS19105 (3913259) 3913259..3913399 - 141 WP_004147370.1 hypothetical protein -
LOX64_RS19110 (3913420) 3913420..3913890 + 471 WP_002892026.1 YlaC family protein -
LOX64_RS19115 (3913865) 3913865..3915316 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LOX64_RS19120 (3915417) 3915417..3916115 + 699 WP_002892021.1 GNAT family protein -
LOX64_RS19125 (3916112) 3916112..3916252 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LOX64_RS19130 (3916252) 3916252..3916515 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224777 WP_002892050.1 NZ_CP087122:3912344-3912562 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T224777 NZ_CP120549:c818171-817797 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT224777 WP_002892066.1 NZ_CP087122:3911943-3912317 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT224777 NZ_CP120549:c818629-818261 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References