Detailed information of TA system TA224775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2576989..2578577
• Replicon: chromosome
• Accession: NZ_CP087122
• Organism: Klebsiella pneumoniae strain TH12852

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LOX64_RS12705
• Protein ID: WP_229982630.1
• Coordinates: 2576989..2578311 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LOX64_RS12710
• Protein ID: WP_002904790.1
• Coordinates: 2578311..2578577 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX64_RS12690 (2572045)	2572045..2574546	+	2502	WP_038807929.1	fimbria/pilus outer membrane usher protein	-
LOX64_RS12695 (2574534)	2574534..2575538	+	1005	WP_004184029.1	fimbrial protein	-
LOX64_RS12700 (2575680)	2575680..2576972	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LOX64_RS12705 (2576989)	2576989..2578311	-	1323	WP_229982630.1	type II toxin-antitoxin system HipA family toxin	Toxin
LOX64_RS12710 (2578311)	2578311..2578577	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LOX64_RS12715 (2578843)	2578843..2579169	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
LOX64_RS12720 (2579166)	2579166..2579666	-	501	WP_032411470.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LOX64_RS12725 (2579644)	2579644..2581023	-	1380	WP_032411468.1	nucleobase:cation symporter-2 family protein	-
LOX64_RS12730 (2581369)	2581369..2582523	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LOX64_RS12735 (2582504)	2582504..2583427	-	924	WP_032411466.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49129.49 Da
• Isoelectric Point: 7.8246

>T224775 WP_229982630.1 NZ_CP087122:c2578311-2576989 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITCQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYGIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T224775 NZ_CP120549:180498-180605 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT224775 WP_002904790.1 NZ_CP087122:c2578577-2578311 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT224775 NZ_CP120549:c180441-180386 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8