Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1728749..1729339 Replicon chromosome
Accession NZ_CP087122
Organism Klebsiella pneumoniae strain TH12852

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag LOX64_RS08335 Protein ID WP_022615588.1
Coordinates 1729007..1729339 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag LOX64_RS08330 Protein ID WP_000288812.1
Coordinates 1728749..1729006 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX64_RS08305 (1724658) 1724658..1724732 + 75 Protein_1626 type II toxin-antitoxin system PemK/MazF family toxin -
LOX64_RS08310 (1725084) 1725084..1726832 + 1749 WP_117008874.1 hypothetical protein -
LOX64_RS08315 (1726911) 1726911..1727468 + 558 WP_087638062.1 hypothetical protein -
LOX64_RS08320 (1727751) 1727751..1728211 + 461 Protein_1629 hypothetical protein -
LOX64_RS08325 (1728208) 1728208..1728414 + 207 WP_020805021.1 helix-turn-helix transcriptional regulator -
LOX64_RS08330 (1728749) 1728749..1729006 + 258 WP_000288812.1 antitoxin Antitoxin
LOX64_RS08335 (1729007) 1729007..1729339 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LOX64_RS08345 (1729661) 1729661..1731097 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LOX64_RS08355 (1731463) 1731463..1732917 - 1455 WP_004148975.1 AMP nucleosidase -
LOX64_RS08360 (1733047) 1733047..1733292 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T224769 WP_022615588.1 NZ_CP087122:1729007-1729339 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T224769 NZ_CP120524:156927-157112 [Enterobacter roggenkampii]
GTGAAAAGTGCGGATGTCATTACTGTTCTGATCAGCCATGGCTGGAAATGTGTCAGAACGAAAGGAAGCCATCATCAGTT
TCGCCATCCCGAACAGAAAGGGCTGGTTACTGTCCCACATCCCAAGAAGGATATTAAACCGGGCACTCTCGCCCAGATCT
GGCGACAGGCGGGTATTAAACACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT224769 WP_000288812.1 NZ_CP087122:1728749-1729006 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT224769 NZ_CP120524:157127-157534 [Enterobacter roggenkampii]
ATGTTTTATCCGGCTTACATTCATTCTGACTCCGACGGTAGCGCCAGCGGCTTTTTCCCTGACGTTCCAGGCTGTTTTTT
TGCAGGTGATTCCCTGGACGACGCTTTTCAGGATGCGCGTGATGCGCTCACAGCACACTTCGAAGCGCTATTTGAAATGG
ATAATGCACTGCCTCTTCCGGGTAACGTGGAAGCGCATCTGGAAAGTCGTCCTGAGGATTTCACTGGCGGGCAATGGCTG
TTGGTAGATATTAATATGAAACAGTTCGACGGTAGGGCCGAGCGTATCAACATCACGATGCCTCGACGTTTGCTGGTAAA
AATTGATTCCTTTGTCAGCGAGCATCCTCAGTTCGGTAATCGAAGCGCATTTCTGGCTGAGGCAGCACGTCGCGTGCTGC
CTGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References