Detailed information of TA system TA224761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-DUF971
• Location: 1242563..1243136
• Replicon: chromosome
• Accession: NZ_CP087114
• Organism: Bartonella machadoae strain 46A

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LNM86_RS05985
• Protein ID: WP_241438487.1
• Coordinates: 1242825..1243136 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LNM86_RS05980
• Protein ID: WP_241438486.1
• Coordinates: 1242563..1242844 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNM86_RS05955 (LNM86_05955)	1237576..1239600	+	2025	WP_241438811.1	portal protein	-
LNM86_RS05960 (LNM86_05960)	1239613..1240512	+	900	WP_241438482.1	hypothetical protein	-
LNM86_RS05965 (LNM86_05965)	1240606..1241736	+	1131	WP_241438483.1	N4-gp56 family major capsid protein	-
LNM86_RS05970 (LNM86_05970)	1241754..1242134	+	381	WP_241438484.1	hypothetical protein	-
LNM86_RS05975 (LNM86_05975)	1242147..1242527	+	381	WP_241438485.1	hypothetical protein	-
LNM86_RS05980 (LNM86_05980)	1242563..1242844	-	282	WP_241438486.1	putative addiction module antidote protein	Antitoxin
LNM86_RS05985 (LNM86_05985)	1242825..1243136	-	312	WP_241438487.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNM86_RS05990 (LNM86_05990)	1243200..1243394	+	195	Protein_1175	hypothetical protein	-
LNM86_RS05995 (LNM86_05995)	1243561..1244727	+	1167	WP_241437300.1	site-specific integrase	-
LNM86_RS06000 (LNM86_06000)	1244946..1245152	+	207	WP_241437891.1	DNA-binding protein	-
LNM86_RS06005 (LNM86_06005)	1245243..1245689	+	447	Protein_1178	hypothetical protein	-
LNM86_RS06010 (LNM86_06010)	1245662..1247278	+	1617	WP_241437662.1	YfjI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1209361..1408772	199411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Uncharacterized protein HI1420 (2-89)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11744.71 Da
• Isoelectric Point: 8.9050

>T224761 WP_241438487.1 NZ_CP087114:c1243136-1242825 [Bartonella machadoae]
MVAIIKTTDEFDEWLEKLKDRKAADIILARLHKIRSGLLGDVKFFHGIGELRIHYGAGYRIYFTQKGNKIIILLCGGDKS
TQSKDIQKALSLLEDLKNEIENL

Nucleotide

• Length: 312 bp

>T224761 NZ_CP120426:c2431104-2430925 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10146.57 Da
• Isoelectric Point: 9.8657

>AT224761 WP_241438486.1 NZ_CP087114:c1242844-1242563 [Bartonella machadoae]
MKLKTFNFEKHLTTPESQEVFLNEAFKTGDAAHIADALGIVARAQNMSALAKETNRERSGLYRSLSKTGDPRLSTLVAVL
SALNLQLSVQSTP

Nucleotide

• Length: 282 bp

>AT224761 NZ_CP120426:c2431727-2431128 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA