Detailed information of TA system TA224757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4072735..4073251
• Replicon: chromosome
• Accession: NZ_CP087111
• Organism: Pseudomonas palleroniana strain X49

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LDL65_RS18760
• Protein ID: WP_244384055.1
• Coordinates: 4072735..4073022 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0X7K790
• Locus tag: LDL65_RS18765
• Protein ID: WP_060753830.1
• Coordinates: 4073012..4073251 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL65_RS18735 (LDL65_18735)	4068305..4068676	+	372	WP_065948914.1	DNA binding protein	-
LDL65_RS18740 (LDL65_18740)	4068716..4069435	-	720	WP_244697940.1	endonuclease NucS	-
LDL65_RS18745 (LDL65_18745)	4069544..4069855	-	312	WP_244697941.1	hypothetical protein	-
LDL65_RS18750 (LDL65_18750)	4069877..4070701	-	825	WP_244697942.1	GIY-YIG nuclease family protein	-
LDL65_RS18755 (LDL65_18755)	4070913..4072709	-	1797	WP_244697943.1	tyrosine-type recombinase/integrase	-
LDL65_RS18760 (LDL65_18760)	4072735..4073022	-	288	WP_244384055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDL65_RS18765 (LDL65_18765)	4073012..4073251	-	240	WP_060753830.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LDL65_RS18770 (LDL65_18770)	4073353..4073481	-	129	WP_060753700.1	hypothetical protein	-
LDL65_RS18775 (LDL65_18775)	4073675..4074901	-	1227	WP_060753699.1	MFS transporter	-
LDL65_RS18780 (LDL65_18780)	4075147..4075686	+	540	WP_244697944.1	hypothetical protein	-
LDL65_RS18785 (LDL65_18785)	4075755..4076633	-	879	WP_244697945.1	hypothetical protein	-
LDL65_RS18790 (LDL65_18790)	4076828..4077730	+	903	WP_060753829.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4004060..4073353	69293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11334.18 Da
• Isoelectric Point: 10.1270

>T224757 WP_244384055.1 NZ_CP087111:c4073022-4072735 [Pseudomonas palleroniana]
MIYSLDFDARALKEWQKLGNTIRQQLKKKLMEILNDPRIEANRLHGLPDCYKIKLRSSGYRLVYQVIDHEITVFVVAVDK
RERDRVYQRAAERLS

Nucleotide

• Length: 288 bp

>T224757 NZ_CP120425:2406932-2407111 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9096.54 Da
• Isoelectric Point: 4.8011

>AT224757 WP_060753830.1 NZ_CP087111:c4073251-4073012 [Pseudomonas palleroniana]
MASINIRIDDDLKARAYLELERLGVTPSELMRQTLQYVVERGQLPFKPVLMTEDDEALLAKVRERLASPQRVRVSIDDL

Nucleotide

• Length: 240 bp

>AT224757 NZ_CP120425:2406309-2406908 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0X7K790