Detailed information of TA system TA224756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 4024045..4024631
• Replicon: chromosome
• Accession: NZ_CP087111
• Organism: Pseudomonas palleroniana strain X49

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6I6W8S7
• Locus tag: LDL65_RS18500
• Protein ID: WP_056788882.1
• Coordinates: 4024323..4024631 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0Q8YRR5
• Locus tag: LDL65_RS18495
• Protein ID: WP_056788879.1
• Coordinates: 4024045..4024326 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL65_RS18465 (LDL65_18465)	4020062..4021210	+	1149	WP_244697867.1	hypothetical protein	-
LDL65_RS18470 (LDL65_18470)	4021295..4021822	+	528	WP_177076067.1	hypothetical protein	-
LDL65_RS18475 (LDL65_18475)	4021822..4022064	+	243	WP_244697869.1	hypothetical protein	-
LDL65_RS18480 (LDL65_18480)	4022082..4022774	+	693	WP_244697871.1	SOS response-associated peptidase family protein	-
LDL65_RS18485 (LDL65_18485)	4022812..4023573	+	762	WP_244697873.1	hypothetical protein	-
LDL65_RS18490 (LDL65_18490)	4023671..4023991	+	321	WP_244697875.1	hypothetical protein	-
LDL65_RS18495 (LDL65_18495)	4024045..4024326	-	282	WP_056788879.1	putative addiction module antidote protein	Antitoxin
LDL65_RS18500 (LDL65_18500)	4024323..4024631	-	309	WP_056788882.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDL65_RS18505 (LDL65_18505)	4024689..4025447	-	759	WP_244697877.1	hypothetical protein	-
LDL65_RS18510 (LDL65_18510)	4025447..4025968	-	522	WP_244697880.1	hypothetical protein	-
LDL65_RS18515 (LDL65_18515)	4025979..4026413	-	435	WP_065948956.1	hypothetical protein	-
LDL65_RS18520 (LDL65_18520)	4026482..4027552	-	1071	WP_073454415.1	hypothetical protein	-
LDL65_RS18525 (LDL65_18525)	4027554..4028165	-	612	WP_073454413.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4004060..4073353	69293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11478.19 Da
• Isoelectric Point: 7.8182

>T224756 WP_056788882.1 NZ_CP087111:c4024631-4024323 [Pseudomonas palleroniana]
MNTIITTHEFDNWLKGLKDVQARLRIVSRILSAEAGNFGDCEPVGEGISEMRIFVGPGYRVYYTRRGEVVYLLLCGGNKS
SQKRDIKLAKSILDEIEQGDVK

Nucleotide

• Length: 309 bp

>T224756 NZ_CP120425:2203701-2204063 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10287.83 Da
• Isoelectric Point: 7.0104

>AT224756 WP_056788879.1 NZ_CP087111:c4024326-4024045 [Pseudomonas palleroniana]
MRELKAFDASQYLTNEDEIAQFLTISLEDPNPDVFLRALQEVAKARGMSQLAKDAGINRESLYKALAPGAKPRYDTVLKL
VNALGVKLTAQHV

Nucleotide

• Length: 282 bp

>AT224756 NZ_CP120425:2203534-2203704 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTTCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A6I6W8S7

Antitoxin

• AlphaFold DB: A0A0Q8YRR5