Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 3113213..3113807 Replicon chromosome
Accession NZ_CP087111
Organism Pseudomonas palleroniana strain X49

Toxin (Protein)


Gene name PumA Uniprot ID A0A0X7K2Q1
Locus tag LDL65_RS14225 Protein ID WP_060755039.1
Coordinates 3113493..3113807 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X7K2N6
Locus tag LDL65_RS14220 Protein ID WP_060755040.1
Coordinates 3113213..3113500 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDL65_RS14200 (LDL65_14200) 3109634..3111694 - 2061 WP_244701802.1 TonB-dependent copper receptor -
LDL65_RS14205 (LDL65_14205) 3111790..3112179 - 390 WP_104995390.1 DUF2946 domain-containing protein -
LDL65_RS14210 (LDL65_14210) 3112191..3112673 - 483 WP_060755042.1 copper chaperone PCu(A)C -
LDL65_RS14215 (LDL65_14215) 3112720..3113115 - 396 WP_244701804.1 DUF2946 domain-containing protein -
LDL65_RS14220 (LDL65_14220) 3113213..3113500 - 288 WP_060755040.1 putative addiction module antidote protein Antitoxin
LDL65_RS14225 (LDL65_14225) 3113493..3113807 - 315 WP_060755039.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LDL65_RS14230 (LDL65_14230) 3114313..3114564 + 252 WP_244701806.1 DUF6124 family protein -
LDL65_RS14235 (LDL65_14235) 3114629..3114952 - 324 WP_046485680.1 transcriptional regulator -
LDL65_RS14240 (LDL65_14240) 3114958..3115290 - 333 WP_104995393.1 addiction module protein -
LDL65_RS14245 (LDL65_14245) 3115347..3116072 - 726 WP_104995394.1 cobalt-precorrin-6A reductase -
LDL65_RS14250 (LDL65_14250) 3116069..3117274 - 1206 WP_104995395.1 precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE -
LDL65_RS14255 (LDL65_14255) 3117313..3118659 + 1347 WP_090367049.1 precorrin-3B synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Uncharacterized protein HI1420

(3-94)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12066.80 Da        Isoelectric Point: 10.0959

>T224755 WP_060755039.1 NZ_CP087111:c3113807-3113493 [Pseudomonas palleroniana]
MIRFKRSAEFQEWFHSLRDKPARGRVLTRLDNATMGNFGDCETVGNGVSEMRIHYGPGYRVYFMRIGEVVYLLLIGGDKS
TQQRDIQRAKQIADEFRKKGVVHD

Download         Length: 315 bp

>T224755 NZ_CP120425:2192651-2192860 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10455.89 Da        Isoelectric Point: 4.8430

>AT224755 WP_060755040.1 NZ_CP087111:c3113500-3113213 [Pseudomonas palleroniana]
MTEFKDFDIAEYLKTPEDMAEYLEASFEEDSGDGLLIRSALNHIARAQGMTQIARDAGLGRESLYKALSSTGNPEFATIM
KVMKALGLKLHASAR

Download         Length: 288 bp

>AT224755 NZ_CP120425:2192351-2192644 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X7K2Q1

Antitoxin

Source ID Structure
AlphaFold DB A0A0X7K2N6

References