Detailed information of TA system TA224748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3891061..3891875
• Replicon: chromosome
• Accession: NZ_CP087110
• Organism: Escherichia coli strain PJ-T13

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: LM520_RS19085
• Protein ID: WP_001054376.1
• Coordinates: 3891061..3891318 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: LM520_RS19090
• Protein ID: WP_001309181.1
• Coordinates: 3891330..3891875 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM520_RS19060 (3886349)	3886349..3887455	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
LM520_RS19065 (3887520)	3887520..3888500	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
LM520_RS19070 (3888610)	3888610..3888815	+	206	Protein_3733	HNH endonuclease	-
LM520_RS19075 (3889083)	3889083..3890323	-	1241	Protein_3734	helicase YjhR	-
LM520_RS19080 (3890439)	3890439..3890570	+	132	WP_001309182.1	hypothetical protein	-
LM520_RS19085 (3891061)	3891061..3891318	+	258	WP_001054376.1	YjhX family toxin	Toxin
LM520_RS19090 (3891330)	3891330..3891875	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
LM520_RS19095 (3891931)	3891931..3892677	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
LM520_RS19100 (3892846)	3892846..3893064	+	219	Protein_3739	hypothetical protein	-
LM520_RS19105 (3893102)	3893102..3893218	+	117	Protein_3740	VOC family protein	-
LM520_RS19110 (3893463)	3893463..3894584	+	1122	WP_000010829.1	M42 family metallopeptidase	-
LM520_RS19115 (3894581)	3894581..3894859	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
LM520_RS19120 (3894871)	3894871..3896184	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	3880847..3900099	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T224748 WP_001054376.1 NZ_CP087110:3891061-3891318 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T224748 NZ_CP120423:c1392186-1391998 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT224748 WP_001309181.1 NZ_CP087110:3891330-3891875 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT224748 NZ_CP120423:c1392468-1392211 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8