Detailed information of TA system TA224742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2235261..2235899
• Replicon: chromosome
• Accession: NZ_CP087110
• Organism: Escherichia coli strain PJ-T13

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LM520_RS10895
• Protein ID: WP_000813794.1
• Coordinates: 2235723..2235899 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LM520_RS10890
• Protein ID: WP_001270286.1
• Coordinates: 2235261..2235677 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM520_RS10870 (2230413)	2230413..2231354	-	942	WP_001251304.1	ABC transporter permease	-
LM520_RS10875 (2231355)	2231355..2232368	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LM520_RS10880 (2232386)	2232386..2233531	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LM520_RS10885 (2233776)	2233776..2235182	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LM520_RS10890 (2235261)	2235261..2235677	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LM520_RS10895 (2235723)	2235723..2235899	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LM520_RS10900 (2236121)	2236121..2236351	+	231	WP_000494244.1	YncJ family protein	-
LM520_RS10905 (2236443)	2236443..2238404	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LM520_RS10910 (2238477)	2238477..2239013	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LM520_RS10915 (2239105)	2239105..2240280	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T224742 WP_000813794.1 NZ_CP087110:c2235899-2235723 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T224742 NZ_CP120399:c4250608-4250117 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGTGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT224742 WP_001270286.1 NZ_CP087110:c2235677-2235261 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT224742 NZ_CP120399:c4250898-4250605 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGTTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGTGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• PDB: 6HPB