Detailed information of TA system TA022474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 4648375..4648885
• Replicon: chromosome
• Accession: NC_009831
• Organism: Shewanella sediminis HAW-EB3

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A8FZZ6
• Locus tag: SSED_RS19935
• Protein ID: WP_012144149.1
• Coordinates: 4648622..4648885 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A8FZZ5
• Locus tag: SSED_RS19930
• Protein ID: WP_012144148.1
• Coordinates: 4648375..4648629 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSED_RS19910	4643738..4644217	-	480	WP_012144144.1	TetR family transcriptional regulator	-
SSED_RS19915	4644614..4645489	-	876	WP_049772138.1	hypothetical protein	-
SSED_RS19920	4645486..4646916	-	1431	WP_012144146.1	bifunctional D-glycero-beta-D-manno-heptose-7-phosphate kinase/D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase HldE	-
SSED_RS19925	4647077..4648003	+	927	WP_012144147.1	LpxL/LpxP family Kdo(2)-lipid IV(A) lauroyl/palmitoleoyl acyltransferase	-
SSED_RS19930	4648375..4648629	+	255	WP_012144148.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
SSED_RS19935	4648622..4648885	+	264	WP_012144149.1	Txe/YoeB family addiction module toxin	Toxin
SSED_RS19940	4648937..4649770	-	834	WP_150104462.1	FAD:protein FMN transferase	-
SSED_RS19945	4649891..4651189	-	1299	WP_012144151.1	DUF3570 domain-containing protein	-
SSED_RS19950	4651180..4651395	-	216	WP_012144152.1	DUF4266 domain-containing protein	-
SSED_RS19955	4651399..4651866	-	468	WP_012144153.1	TlpA family protein disulfide reductase	-
SSED_RS19960	4652192..4653373	-	1182	WP_012144154.1	flagellin lysine-N-methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10395.85 Da
• Isoelectric Point: 8.8578

>T22474 WP_012144149.1 NC_009831:4648622-4648885 [Shewanella sediminis HAW-EB3]
MSSRLLSWTDDAWNDYLYWQTQDKKTLKRINKLINDVKRLPFEGIGKPEPLKENLAGFWSRRIDDTNRLVYAVDDSVITI
ISCRYHY

Nucleotide

• Length: 264 bp

>T22474 NC_009831:4648622-4648885 [Shewanella sediminis HAW-EB3]
ATGAGTAGTCGTTTATTGTCATGGACTGATGATGCCTGGAACGACTATCTGTACTGGCAAACGCAAGATAAGAAAACACT
GAAACGCATCAATAAACTCATCAATGATGTGAAGCGTTTACCTTTTGAGGGTATTGGAAAACCTGAGCCTCTGAAAGAAA
ACTTAGCGGGTTTCTGGTCTCGGCGCATCGATGATACTAACCGCTTAGTGTACGCCGTTGACGATAGTGTAATTACGATA
ATCTCCTGTCGTTATCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9481.66 Da
• Isoelectric Point: 5.0623

>AT22474 WP_012144148.1 NC_009831:4648375-4648629 [Shewanella sediminis HAW-EB3]
MRIISFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLNRSIAQYRSGKVTQRE
LIDE

Nucleotide

• Length: 255 bp

>AT22474 NC_009831:4648375-4648629 [Shewanella sediminis HAW-EB3]
ATGAGAATCATATCTTTTACAGAAGCACGAAATGGCTTGAAAGCAGTATTGGACGGTGTGGTTAATGATGCTGACACGAC
TGTAATTACACGCAGAGATTCTGAAGATGCAGTTGTTATGTCACTGGACTACTACAACAGTCTAATGGAAACGGTGCACT
TGCTTCGCTCTCCGGCAAACGCAGAGCATCTGAATCGTTCAATTGCTCAATATAGATCGGGAAAAGTAACTCAAAGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: A8FZZ6

Antitoxin

• AlphaFold DB: A8FZZ5