Detailed information of TA system TA224731

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1665344..1666175
• Replicon: chromosome
• Accession: NZ_CP087110
• Organism: Escherichia coli strain PJ-T13

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: LM520_RS07985
• Protein ID: WP_000854814.1
• Coordinates: 1665344..1665718 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: LM520_RS07990
• Protein ID: WP_001285584.1
• Coordinates: 1665807..1666175 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM520_RS07945 (1660740)	1660740..1661906	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
LM520_RS07950 (1662025)	1662025..1662498	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
LM520_RS07955 (1662696)	1662696..1663754	+	1059	WP_001200891.1	FUSC family protein	-
LM520_RS07960 (1663926)	1663926..1664255	+	330	WP_000450409.1	DUF496 family protein	-
LM520_RS07965 (1664356)	1664356..1664490	-	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
LM520_RS07970 (1664610)	1664610..1664738	+	129	Protein_1558	transposase domain-containing protein	-
LM520_RS07975 (1665027)	1665027..1665107	-	81	Protein_1559	hypothetical protein	-
LM520_RS07980 (1665153)	1665153..1665347	-	195	WP_000988600.1	DUF5983 family protein	-
LM520_RS07985 (1665344)	1665344..1665718	-	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
LM520_RS07990 (1665807)	1665807..1666175	-	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
LM520_RS07995 (1666249)	1666249..1666470	-	222	WP_000692323.1	DUF987 domain-containing protein	-
LM520_RS08000 (1666533)	1666533..1666979	-	447	WP_000187523.1	RadC family protein	-
LM520_RS08005 (1666976)	1666976..1668508	-	1533	WP_001350525.1	protein YeeR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T224731 WP_000854814.1 NZ_CP087110:c1665718-1665344 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T224731 NZ_CP120398:c4676343-4675969 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT224731 WP_001285584.1 NZ_CP087110:c1666175-1665807 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT224731 NZ_CP120398:c4676585-4676343 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6