Detailed information of TA system TA022473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 439843..441382
• Replicon: chromosome
• Accession: NC_009831
• Organism: Shewanella sediminis HAW-EB3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A8FQ65
• Locus tag: SSED_RS01930
• Protein ID: WP_012140726.1
• Coordinates: 439843..441147 (-)
• Length: 435 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: SSED_RS01935
• Protein ID: WP_012140727.1
• Coordinates: 441134..441382 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSED_RS01910	435056..436882	+	1827	WP_012140722.1	hypothetical protein	-
SSED_RS01915	437011..438036	+	1026	WP_012140723.1	manganese-dependent inorganic pyrophosphatase	-
SSED_RS01920	438266..438928	+	663	WP_012140724.1	adenylate kinase	-
SSED_RS01925	439043..439681	-	639	WP_012140725.1	multidrug MFS transporter	-
SSED_RS01930	439843..441147	-	1305	WP_012140726.1	HipA domain-containing protein	Toxin
SSED_RS01935	441134..441382	-	249	WP_012140727.1	helix-turn-helix domain-containing protein	Antitoxin
SSED_RS01940	441797..442660	-	864	WP_012140728.1	YicC family protein	-
SSED_RS01945	442841..443554	+	714	WP_012140729.1	ribonuclease PH	-
SSED_RS01950	443641..444282	+	642	WP_012140730.1	orotate phosphoribosyltransferase	-
SSED_RS01955	444582..445241	-	660	WP_012140731.1	GTP cyclohydrolase I FolE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (13-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 435 a.a.
• Molecular weight: 49168.40 Da
• Isoelectric Point: 5.7644

>T22473 WP_012140726.1 NC_009831:c441147-439843 [Shewanella sediminis HAW-EB3]
MSGSNFHILEMHFRGMVIGAIGFDIDTEQFSIEYTKPWQEKGFPISPRLPLDGQASSSQISIYLTNLLPENKGLDHLIEA
LGIAKSNTFGLIKGIGFDTSGAILYLPVGTKPPATSFTPICKEDLIKRIEDPEHWPMEVWDGKPRLSVAGVQSKLNLFHS
DKEYGFGEGELCSTHIIKFEKNNQHLVLNEFITMTLAGLIGMQVAKVDIMPIGKYKALSVTRFDRMYEPERHIVMRRHQI
DVCQALGFSVSRKYERNFGSGRDVQDIREGVSLERLFSIAEHCANPAQAKFDMLRWTIFNLLVCNADSHGKNYSFYVTPD
GLEPTPWYDLVNVQMYPDFDQELAMAIGDEFEADKIHAYQLMTFAEECGIKQNILELSLKQLAKAIIGNLDKAAKQTGEI
TDAESKYVETYKKTIQARCEYYLAQVEEMKHIKL

Nucleotide

• Length: 1305 bp

>T22473 NC_009831:c441147-439843 [Shewanella sediminis HAW-EB3]
ATGAGTGGTTCTAACTTTCACATCCTAGAGATGCACTTTAGAGGCATGGTTATTGGGGCGATAGGTTTTGACATAGACAC
TGAACAGTTCTCTATCGAATACACTAAACCTTGGCAGGAAAAGGGCTTTCCAATTTCACCAAGGCTCCCACTTGATGGCC
AAGCCAGCAGTTCTCAAATTTCCATTTACCTGACTAACCTACTGCCCGAAAATAAAGGGCTTGATCACCTCATTGAAGCC
CTAGGCATTGCCAAAAGTAATACATTTGGTCTGATCAAGGGCATTGGGTTTGATACCTCTGGTGCAATATTGTATCTGCC
TGTGGGCACCAAACCACCAGCAACCTCTTTTACTCCGATTTGCAAAGAAGACCTCATCAAACGAATCGAAGATCCTGAAC
ATTGGCCGATGGAAGTCTGGGACGGAAAACCACGCCTTTCAGTTGCTGGAGTTCAATCAAAATTAAACCTATTCCACAGC
GACAAAGAATACGGATTTGGTGAAGGTGAACTCTGCTCAACCCACATCATTAAATTTGAAAAAAACAATCAGCACCTCGT
ACTTAACGAATTTATTACCATGACACTTGCCGGCCTCATCGGCATGCAGGTTGCGAAAGTTGATATAATGCCAATAGGGA
AATACAAAGCATTATCCGTTACCCGCTTCGATCGCATGTACGAACCTGAGCGCCATATTGTAATGCGTCGTCACCAGATC
GATGTATGTCAGGCTCTGGGATTTTCCGTCTCAAGAAAGTATGAACGAAATTTTGGTAGTGGACGTGACGTTCAAGATAT
TCGTGAAGGAGTGAGTCTAGAAAGGCTGTTTAGCATCGCTGAGCACTGTGCTAATCCAGCACAAGCCAAGTTTGACATGC
TTCGTTGGACAATCTTTAACTTACTCGTCTGCAATGCGGACTCACATGGCAAAAACTATTCATTCTATGTAACTCCTGAT
GGGCTTGAACCGACCCCATGGTATGATCTTGTCAACGTGCAGATGTACCCTGATTTTGATCAAGAGCTTGCTATGGCTAT
TGGTGATGAGTTCGAAGCTGACAAGATCCACGCCTACCAATTGATGACATTTGCTGAAGAATGTGGAATTAAGCAAAACA
TACTTGAATTATCGCTCAAGCAACTGGCGAAAGCGATTATCGGCAATCTGGATAAAGCGGCAAAACAAACCGGGGAGATT
ACGGATGCAGAATCCAAGTATGTTGAGACGTACAAAAAAACCATTCAAGCACGATGTGAATACTACCTGGCTCAGGTTGA
AGAAATGAAACACATTAAGCTATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9146.64 Da
• Isoelectric Point: 10.0606

>AT22473 WP_012140727.1 NC_009831:c441382-441134 [Shewanella sediminis HAW-EB3]
MLTQFDPNTLGKLIKDRRKAADMNQEIAASFCGVTKKTLIRVEKGEDVYFSTIIKILNGLGIKLTVETPNSKNKLGGSNE
WF

Nucleotide

• Length: 249 bp

>AT22473 NC_009831:c441382-441134 [Shewanella sediminis HAW-EB3]
ATGCTCACTCAATTCGATCCAAACACCTTAGGCAAACTAATCAAAGACCGCCGAAAGGCCGCTGACATGAATCAGGAAAT
AGCCGCATCATTCTGTGGTGTTACCAAGAAGACCTTGATCAGAGTCGAAAAAGGCGAAGATGTGTACTTTTCAACGATAA
TCAAAATCCTCAATGGCCTTGGTATAAAGCTGACCGTCGAGACTCCTAATAGCAAAAACAAATTAGGTGGCAGCAATGAG
TGGTTCTAA

Structures

Toxin

• AlphaFold DB: A8FQ65