Detailed information of TA system TA224722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 298013..298703
• Replicon: plasmid pNMS14P1
• Accession: NZ_CP087107
• Organism: Methylobacterium sp. NMS14P

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOK46_RS31240
• Protein ID: WP_273564772.1
• Coordinates: 298257..298703 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOK46_RS31235
• Protein ID: WP_273565221.1
• Coordinates: 298013..298252 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOK46_RS31210 (LOK46_31150)	293598..293846	-	249	WP_273564767.1	hypothetical protein	-
LOK46_RS31215 (LOK46_31155)	294472..294735	-	264	WP_273564768.1	hypothetical protein	-
LOK46_RS31220 (LOK46_31160)	295364..295639	+	276	WP_273564769.1	GNAT family N-acetyltransferase	-
LOK46_RS31225 (LOK46_31165)	295724..297049	-	1326	WP_273564770.1	TAXI family TRAP transporter solute-binding subunit	-
LOK46_RS31230 (LOK46_31170)	297138..297746	-	609	WP_273564771.1	PAS domain-containing protein	-
LOK46_RS31235 (LOK46_31175)	298013..298252	+	240	WP_273565221.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOK46_RS31240 (LOK46_31180)	298257..298703	+	447	WP_273564772.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOK46_RS31245 (LOK46_31185)	298758..299369	-	612	WP_273564773.1	TetR/AcrR family transcriptional regulator	-
LOK46_RS31250 (LOK46_31190)	299595..300680	+	1086	WP_273564774.1	acetylhydrolase	-
LOK46_RS31255 (LOK46_31195)	300767..301330	-	564	WP_273564775.1	TetR/AcrR family transcriptional regulator	-
LOK46_RS31260 (LOK46_31200)	301433..302638	+	1206	WP_273564776.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..542519	542519

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-64)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15792.20 Da
• Isoelectric Point: 7.3854

>T224722 WP_273564772.1 NZ_CP087107:298257-298703 [Methylobacterium sp. NMS14P]
VYLVDTNVLSAGAPTKAVPAVELIDWMDRNSAGLHLSVVTIAEIEDGIAKSRREGAARKAERLAEWLETLLHLYAAKVLP
IDLAVARHIGGLSDLARGQGHAPGFADLAIAATARCNGWTVLTRNVRHFAPLGVPACDPFVALPADRR

Nucleotide

• Length: 447 bp

>T224722 NZ_CP120398:805693-806094 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCCTTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGACAG
GACTGGACGCTCTTGCGTCCTCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
AG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8583.79 Da
• Isoelectric Point: 5.7735

>AT224722 WP_273565221.1 NZ_CP087107:298013-298252 [Methylobacterium sp. NMS14P]
MQEIQLRDAKATLSAVIDQAKQGEPSIITRHGRPEAVLLSFEDWQRLSRVPSFGRLLMAAPVAADDLPSPSGGLRSSEL

Nucleotide

• Length: 240 bp

>AT224722 NZ_CP120398:805446-805712 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA