Detailed information of TA system TA224721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1250854..1251494
• Replicon: chromosome
• Accession: NZ_CP087106
• Organism: Methylobacterium sp. NMS14P

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOK46_RS05890
• Protein ID: WP_273562911.1
• Coordinates: 1251093..1251494 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOK46_RS05885
• Protein ID: WP_273562910.1
• Coordinates: 1250854..1251093 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOK46_RS05870 (LOK46_05875)	1246286..1246522	-	237	WP_007568343.1	BolA family transcriptional regulator	-
LOK46_RS05875 (LOK46_05880)	1246549..1248774	-	2226	WP_273562908.1	phosphoribosylformylglycinamidine synthase subunit PurL	-
LOK46_RS05880 (LOK46_05885)	1248929..1250770	-	1842	WP_273562909.1	DNA helicase RecQ	-
LOK46_RS05885 (LOK46_05890)	1250854..1251093	+	240	WP_273562910.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LOK46_RS05890 (LOK46_05895)	1251093..1251494	+	402	WP_273562911.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOK46_RS05895 (LOK46_05900)	1251627..1252604	-	978	WP_273562912.1	SDR family oxidoreductase	-
LOK46_RS05900 (LOK46_05905)	1252631..1253905	-	1275	WP_273562913.1	four-carbon acid sugar kinase family protein	-
LOK46_RS05905 (LOK46_05910)	1253902..1254684	-	783	WP_273562914.1	hydroxypyruvate isomerase family protein	-
LOK46_RS05910 (LOK46_05915)	1254698..1255606	-	909	WP_273562915.1	NAD-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-122)

Antitoxin

• Antitoxin VapB-like (1-75)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14280.41 Da
• Isoelectric Point: 6.0764

>T224721 WP_273562911.1 NZ_CP087106:1251093-1251494 [Methylobacterium sp. NMS14P]
MFVDASAIVAIVTGEPGHRTLLDRLRDGSVRLTSGVAVYEAVLAIAWLQAVPPEVAREIVDDFLDAAGVTRVPIAEAEAR
LAVMAHARYGKGRHPARLNLGDCFAYAFARVHGVPLLYVGDEFPQTDIRSALG

Nucleotide

• Length: 402 bp

>T224721 NZ_CP120398:204780-205265 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGACGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8895.92 Da
• Isoelectric Point: 4.6283

>AT224721 WP_273562910.1 NZ_CP087106:1250854-1251093 [Methylobacterium sp. NMS14P]
MPLNIRSEEVNQLAEKLASRAGVSKTEAVRLALANELARRDAERETFLERVTQIQERLASYPSTGLQADKAFYDSLNDE

Nucleotide

• Length: 240 bp

>AT224721 NZ_CP120398:204505-204792 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAGTCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA