Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 181616..182157 Replicon plasmid P3
Accession NZ_CP087093
Organism Pseudosulfitobacter pseudonitzschiae strain SC1-26

Toxin (Protein)


Gene name mazF Uniprot ID A0A073IW87
Locus tag LOE12_RS22130 Protein ID WP_037931244.1
Coordinates 181616..181942 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A073IWP4
Locus tag LOE12_RS22135 Protein ID WP_037931247.1
Coordinates 181939..182157 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOE12_RS22100 (LOE12_22100) 176910..177407 - 498 WP_037931962.1 DUF5519 family protein -
LOE12_RS22105 (LOE12_22105) 177416..178231 - 816 WP_224556226.1 class III extradiol ring-cleavage dioxygenase -
LOE12_RS22110 (LOE12_22110) 178295..178702 - 408 WP_157802081.1 DoxX family protein -
LOE12_RS22115 (LOE12_22115) 178865..179188 + 324 WP_027238707.1 helix-turn-helix domain-containing protein -
LOE12_RS22120 (LOE12_22120) 179524..181096 + 1573 Protein_177 IS21 family transposase -
LOE12_RS22125 (LOE12_22125) 181086..181442 + 357 Protein_178 ATP-binding protein -
LOE12_RS22130 (LOE12_22130) 181616..181942 - 327 WP_037931244.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LOE12_RS22135 (LOE12_22135) 181939..182157 - 219 WP_037931247.1 antitoxin MazE family protein Antitoxin
LOE12_RS22140 (LOE12_22140) 182455..183588 - 1134 WP_037931249.1 alpha/beta fold hydrolase -
LOE12_RS22145 (LOE12_22145) 183592..184056 - 465 WP_051694690.1 MaoC/PaaZ C-terminal domain-containing protein -
LOE12_RS22150 (LOE12_22150) 184111..185658 - 1548 WP_073194592.1 AMP-binding protein -
LOE12_RS22155 (LOE12_22155) 185893..186900 - 1008 WP_081855484.1 PaaX family transcriptional regulator C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..290591 290591
- flank IS/Tn - - 179810..181096 1286


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-105)

Antitoxin

Antitoxin MazE-like

(7-71)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11610.50 Da        Isoelectric Point: 8.8545

>T224715 WP_037931244.1 NZ_CP087093:c181942-181616 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Download         Length: 327 bp

>T224715 NZ_CP120397:c2734079-2733977 [Salmonella enterica subsp. enterica serovar Typhi]
GCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8175.17 Da        Isoelectric Point: 5.8017

>AT224715 WP_037931247.1 NZ_CP087093:c182157-181939 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Download         Length: 219 bp

>AT224715 NZ_CP120397:2733975-2734118 [Salmonella enterica subsp. enterica serovar Typhi]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A073IW87

Antitoxin

Source ID Structure
AlphaFold DB A0A073IWP4

References