Detailed information of TA system TA022471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4926632..4927208
• Replicon: chromosome
• Accession: NC_009801
• Organism: Escherichia coli E24377A

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: ECE24377A_RS26130
• Protein ID: WP_001297643.1
• Coordinates: 4926632..4926919 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A080I7H7
• Locus tag: ECE24377A_RS26135
• Protein ID: WP_000063160.1
• Coordinates: 4926906..4927208 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE24377A_RS26110	4922870..4924150	-	1281	WP_001037391.1	DUF445 domain-containing protein	-
ECE24377A_RS26115	4924191..4925423	-	1233	WP_001137031.1	multidrug efflux MFS transporter MdtM	-
ECE24377A_RS29730	4925737..4926433	+	697	Protein_4623	IS1 family transposase	-
ECE24377A_RS26130	4926632..4926919	+	288	WP_001297643.1	BrnT family toxin	Toxin
ECE24377A_RS26135	4926906..4927208	+	303	WP_000063160.1	BrnA antitoxin family protein	Antitoxin
ECE24377A_RS26140	4927242..4928198	-	957	WP_001297640.1	GTPase	-
ECE24377A_RS26145	4928209..4928412	-	204	WP_000467859.1	YbdD/YjiX family protein	-
ECE24377A_RS26150	4928462..4930612	-	2151	WP_001340937.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4926224..4926433	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T22471 WP_001297643.1 NC_009801:4926632-4926919 [Escherichia coli O139:H28 str. E24377A]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T22471 NC_009801:4926632-4926919 [Escherichia coli O139:H28 str. E24377A]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11552.01 Da
• Isoelectric Point: 9.4646

>AT22471 WP_000063160.1 NC_009801:4926906-4927208 [Escherichia coli O139:H28 str. E24377A]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT22471 NC_009801:4926906-4927208 [Escherichia coli O139:H28 str. E24377A]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A080I7H7