Detailed information of TA system TA224708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 7190..7802
• Replicon: plasmid P2
• Accession: NZ_CP087085
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE33_RS20720
• Protein ID: WP_028288627.1
• Coordinates: 7190..7585 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE33_RS20725
• Protein ID: WP_009503866.1
• Coordinates: 7575..7802 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE33_RS20700 (LOE33_20675)	3709..4320	-	612	WP_197919471.1	SMC-Scp complex subunit ScpB	-
LOE33_RS20705 (LOE33_20680)	4368..5252	-	885	WP_037239259.1	DUF1403 family protein	-
LOE33_RS20710 (LOE33_20685)	5379..6518	+	1140	WP_229940649.1	tyrosine-type recombinase/integrase	-
LOE33_RS20715 (LOE33_20690)	6620..7111	+	492	Protein_5	recombinase family protein	-
LOE33_RS20720 (LOE33_20695)	7190..7585	-	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE33_RS20725 (LOE33_20700)	7575..7802	-	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE33_RS20730 (LOE33_20705)	8011..9204	+	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE33_RS20735 (LOE33_20710)	9188..10120	+	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE33_RS20740 (LOE33_20715)	10334..11545	+	1212	WP_050525470.1	plasmid replication protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..197672	197672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224708 WP_028288627.1 NZ_CP087085:c7585-7190 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224708 NZ_CP120397:205084-205569 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGACGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224708 WP_009503866.1 NZ_CP087085:c7802-7575 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224708 NZ_CP120397:204809-205096 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAGTCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA