Detailed information of TA system TA224704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1906072..1906537
• Replicon: chromosome
• Accession: NZ_CP087083
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE33_RS09695
• Protein ID: WP_224557762.1
• Coordinates: 1906072..1906335 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE33_RS09700
• Protein ID: WP_224557764.1
• Coordinates: 1906322..1906537 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE33_RS09670 (LOE33_09675)	1901789..1903339	-	1551	WP_224557015.1	restriction endonuclease subunit S	-
LOE33_RS09675 (LOE33_09680)	1903336..1903632	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE33_RS09680 (LOE33_09685)	1903526..1904014	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE33_RS09685 (LOE33_09690)	1904240..1905348	-	1109	Protein_1917	IS110 family transposase	-
LOE33_RS09690 (LOE33_09695)	1905441..1905884	-	444	WP_224557760.1	hypothetical protein	-
LOE33_RS09695 (LOE33_09700)	1906072..1906335	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE33_RS09700 (LOE33_09705)	1906322..1906537	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE33_RS09705 (LOE33_09710)	1906890..1907114	-	225	WP_224557765.1	hypothetical protein	-
LOE33_RS09710 (LOE33_09715)	1907282..1907662	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE33_RS09715 (LOE33_09720)	1907843..1908814	-	972	WP_224557767.1	hypothetical protein	-
LOE33_RS09720 (LOE33_09725)	1908807..1909991	-	1185	WP_224557768.1	site-specific integrase	-
LOE33_RS09730 (LOE33_09735)	1910369..1911067	-	699	WP_037931342.1	SURF1 family protein	-
LOE33_RS09735 (LOE33_09740)	1911064..1911429	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1897479..1909991	12512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224704 WP_224557762.1 NZ_CP087083:1906072-1906335 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224704 NZ_CP120396:c3965503-3965219 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224704 WP_224557764.1 NZ_CP087083:1906322-1906537 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224704 NZ_CP120396:c3965735-3965493 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA