Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PemK-MazE |
| Location | 119303..119844 | Replicon | plasmid P2 |
| Accession | NZ_CP087075 | ||
| Organism | Pseudosulfitobacter pseudonitzschiae strain MC36-14 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A073IW87 |
| Locus tag | LOE32_RS20265 | Protein ID | WP_037931244.1 |
| Coordinates | 119303..119629 (-) | Length | 109 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A073IWP4 |
| Locus tag | LOE32_RS20270 | Protein ID | WP_037931247.1 |
| Coordinates | 119626..119844 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOE32_RS20235 (LOE32_20235) | 114597..115094 | - | 498 | WP_037931962.1 | DUF5519 family protein | - |
| LOE32_RS20240 (LOE32_20240) | 115103..115918 | - | 816 | WP_224556226.1 | class III extradiol ring-cleavage dioxygenase | - |
| LOE32_RS20245 (LOE32_20245) | 115982..116389 | - | 408 | WP_157802081.1 | DoxX family protein | - |
| LOE32_RS20250 (LOE32_20250) | 116552..116875 | + | 324 | WP_027238707.1 | helix-turn-helix domain-containing protein | - |
| LOE32_RS20255 (LOE32_20255) | 117211..118783 | + | 1573 | Protein_120 | IS21 family transposase | - |
| LOE32_RS20260 (LOE32_20260) | 118773..119129 | + | 357 | Protein_121 | ATP-binding protein | - |
| LOE32_RS20265 (LOE32_20265) | 119303..119629 | - | 327 | WP_037931244.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LOE32_RS20270 (LOE32_20270) | 119626..119844 | - | 219 | WP_037931247.1 | antitoxin MazE family protein | Antitoxin |
| LOE32_RS20275 (LOE32_20275) | 120142..121275 | - | 1134 | WP_037931249.1 | alpha/beta fold hydrolase | - |
| LOE32_RS20280 (LOE32_20280) | 121279..121743 | - | 465 | WP_051694690.1 | MaoC/PaaZ C-terminal domain-containing protein | - |
| LOE32_RS20285 (LOE32_20285) | 121798..123345 | - | 1548 | WP_073194592.1 | AMP-binding protein | - |
| LOE32_RS20290 (LOE32_20290) | 123580..124587 | - | 1008 | WP_081855484.1 | PaaX family transcriptional regulator C-terminal domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..290597 | 290597 | |
| - | flank | IS/Tn | - | - | 117497..118783 | 1286 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 109 a.a. Molecular weight: 11610.50 Da Isoelectric Point: 8.8545
>T224700 WP_037931244.1 NZ_CP087075:c119629-119303 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG
Download Length: 327 bp
>T224700 NZ_CP120396:c953670-953143 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8175.17 Da Isoelectric Point: 5.8017
>AT224700 WP_037931247.1 NZ_CP087075:c119844-119626 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE
Download Length: 219 bp
>AT224700 NZ_CP120396:c953957-953667 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGATTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGATTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IW87 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A073IWP4 |