Detailed information of TA system TA022470

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4885256..4886088
• Replicon: chromosome
• Accession: NC_009801
• Organism: Escherichia coli E24377A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: ECE24377A_RS25920
• Protein ID: WP_000854765.1
• Coordinates: 4885714..4886088 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: ECE24377A_RS25915
• Protein ID: WP_001295723.1
• Coordinates: 4885256..4885624 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE24377A_RS25875	4882371..4882529	+	159	WP_001323397.1	DUF905 family protein	-
ECE24377A_RS25885	4882684..4883502	+	819	WP_001234652.1	DUF945 domain-containing protein	-
ECE24377A_RS25895	4883844..4884317	+	474	WP_000855059.1	antirestriction protein	-
ECE24377A_RS25900	4884333..4884809	+	477	WP_001186775.1	RadC family protein	-
ECE24377A_RS25905	4884872..4885093	+	222	WP_000692345.1	DUF987 domain-containing protein	-
ECE24377A_RS25915	4885256..4885624	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECE24377A_RS25920	4885714..4886088	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
ECE24377A_RS25925	4886085..4886576	+	492	WP_000976842.1	hypothetical protein	-
ECE24377A_RS25930	4886593..4886769	+	177	WP_000839290.1	DUF957 domain-containing protein	-
ECE24377A_RS30640	4886869..4887027	+	159	WP_001467148.1	hypothetical protein	-
ECE24377A_RS25935	4887680..4890571	+	2892	WP_000580534.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T22470 WP_000854765.1 NC_009801:4885714-4886088 [Escherichia coli O139:H28 str. E24377A]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T22470 NC_009801:4885714-4886088 [Escherichia coli O139:H28 str. E24377A]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT22470 WP_001295723.1 NC_009801:4885256-4885624 [Escherichia coli O139:H28 str. E24377A]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT22470 NC_009801:4885256-4885624 [Escherichia coli O139:H28 str. E24377A]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52