Detailed information of TA system TA224694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 162060..162734
• Replicon: plasmid P6
• Accession: NZ_CP087072
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE34_RS23610
• Protein ID: WP_224557924.1
• Coordinates: 162312..162734 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE34_RS23605
• Protein ID: WP_229939240.1
• Coordinates: 162060..162308 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE34_RS23585 (LOE34_23575)	157973..158617	+	645	WP_224557929.1	type IV secretion system protein	-
LOE34_RS23590 (LOE34_23580)	158621..159352	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE34_RS23595 (LOE34_23585)	159355..160812	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE34_RS23600 (LOE34_23590)	160923..161912	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE34_RS23605 (LOE34_23595)	162060..162308	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE34_RS23610 (LOE34_23600)	162312..162734	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE34_RS23615 (LOE34_23605)	162731..165481	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE34_RS23620 (LOE34_23610)	165478..166536	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290595	290595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224694 WP_224557924.1 NZ_CP087072:162312-162734 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224694 NZ_CP120395:4677926-4678237 [Salmonella enterica subsp. enterica serovar Typhi]
GTGCATGTTATCAGCCGAAAACTGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCGCTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATGAAGAATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224694 WP_229939240.1 NZ_CP087072:162060-162308 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224694 NZ_CP120395:4678234-4678680 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG