Detailed information of TA system TA224690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3310129..3310594
• Replicon: chromosome
• Accession: NZ_CP087066
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-22

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE34_RS16715
• Protein ID: WP_224557762.1
• Coordinates: 3310129..3310392 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE34_RS16720
• Protein ID: WP_224557764.1
• Coordinates: 3310379..3310594 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE34_RS16685 (LOE34_16680)	3305214..3306086	-	873	WP_229944613.1	HNH endonuclease family protein	-
LOE34_RS16690 (LOE34_16685)	3306061..3307395	-	1335	WP_229944615.1	restriction endonuclease subunit S	-
LOE34_RS16695 (LOE34_16690)	3307392..3307688	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE34_RS16700 (LOE34_16695)	3307582..3308070	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE34_RS16705 (LOE34_16700)	3308296..3309405	-	1110	WP_224558030.1	IS110 family transposase	-
LOE34_RS16710 (LOE34_16705)	3309498..3309941	-	444	WP_224557760.1	hypothetical protein	-
LOE34_RS16715 (LOE34_16710)	3310129..3310392	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE34_RS16720 (LOE34_16715)	3310379..3310594	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE34_RS16725 (LOE34_16720)	3310947..3311171	-	225	WP_224557765.1	hypothetical protein	-
LOE34_RS16730 (LOE34_16725)	3311339..3311719	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE34_RS16735 (LOE34_16730)	3311900..3312871	-	972	WP_224557767.1	hypothetical protein	-
LOE34_RS16740 (LOE34_16735)	3312864..3314048	-	1185	WP_224557768.1	site-specific integrase	-
LOE34_RS16750 (LOE34_16745)	3314426..3315124	-	699	WP_037931342.1	SURF1 family protein	-
LOE34_RS16755 (LOE34_16750)	3315121..3315486	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224690 WP_224557762.1 NZ_CP087066:3310129-3310392 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224690 NZ_CP120395:c4006708-4006424 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224690 WP_224557764.1 NZ_CP087066:3310379-3310594 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224690 NZ_CP120395:c4006940-4006698 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA