Detailed information of TA system TA224684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 43938..44403
• Replicon: chromosome
• Accession: NZ_CP087059
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-24

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE35_RS00225
• Protein ID: WP_224557762.1
• Coordinates: 44140..44403 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE35_RS00220
• Protein ID: WP_224557764.1
• Coordinates: 43938..44153 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE35_RS00185 (LOE35_00185)	39046..39411	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE35_RS00190 (LOE35_00190)	39408..40106	+	699	WP_037931342.1	SURF1 family protein	-
LOE35_RS00200 (LOE35_00200)	40484..41668	+	1185	WP_224557768.1	site-specific integrase	-
LOE35_RS00205 (LOE35_00205)	41661..42632	+	972	WP_224557767.1	hypothetical protein	-
LOE35_RS00210 (LOE35_00210)	42813..43193	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE35_RS00215 (LOE35_00215)	43361..43585	+	225	WP_224557765.1	hypothetical protein	-
LOE35_RS00220 (LOE35_00220)	43938..44153	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE35_RS00225 (LOE35_00225)	44140..44403	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE35_RS00230 (LOE35_00230)	44591..45034	+	444	WP_224557760.1	hypothetical protein	-
LOE35_RS00235 (LOE35_00235)	45127..46236	+	1110	WP_224558030.1	IS110 family transposase	-
LOE35_RS00240 (LOE35_00240)	46462..46950	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE35_RS00245 (LOE35_00245)	46874..47140	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE35_RS00250 (LOE35_00250)	47137..48687	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224684 WP_224557762.1 NZ_CP087059:c44403-44140 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224684 NZ_CP120395:805693-806094 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCCTTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGACAG
GACTGGACGCTCTTGCGTCCTCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
AG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224684 WP_224557764.1 NZ_CP087059:c44153-43938 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224684 NZ_CP120395:805446-805712 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA