Detailed information of TA system TA224680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 30909..31450
• Replicon: plasmid P3
• Accession: NZ_CP087054
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-43

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE36_RS20430
• Protein ID: WP_037931244.1
• Coordinates: 31124..31450 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE36_RS20425
• Protein ID: WP_037931247.1
• Coordinates: 30909..31127 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE36_RS20405 (LOE36_20420)	26166..27173	+	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-
LOE36_RS20410 (LOE36_20425)	27408..28955	+	1548	WP_073194592.1	AMP-binding protein	-
LOE36_RS20415 (LOE36_20430)	29010..29474	+	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE36_RS20420 (LOE36_20435)	29478..30611	+	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE36_RS20425 (LOE36_20440)	30909..31127	+	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE36_RS20430 (LOE36_20445)	31124..31450	+	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE36_RS20435 (LOE36_20450)	31624..31980	-	357	Protein_36	ATP-binding protein	-
LOE36_RS20440 (LOE36_20455)	31970..33542	-	1573	Protein_37	IS21 family transposase	-
LOE36_RS20445 (LOE36_20460)	33878..34201	-	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE36_RS20450 (LOE36_20465)	34364..34771	+	408	WP_157802081.1	DoxX family protein	-
LOE36_RS20455 (LOE36_20470)	34835..35650	+	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE36_RS20460 (LOE36_20475)	35659..36156	+	498	WP_037931962.1	DUF5519 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290596	290596
	-	flank	IS/Tn	-	-	31970..33256	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224680 WP_037931244.1 NZ_CP087054:31124-31450 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224680 NZ_CP087054:31124-31450 [Pseudosulfitobacter pseudonitzschiae]
GTGAGGCGTGGTGAGCTGGTGACGGTCGCCCTTCAGGGCGACCACGGCAAGCCGCGTCCCGCGCTTGTGATCCAATCCGA
CCGGTTTCCGGACACCGCCACCACGACCGTCCTTCTGGTTACATCCACCTTGGTGGATGCGCCGTTGATTCGGCTGACGG
TCGAACCCTCTGCGGAGAACGGGTTGCGTATGCGATCACAGATCATGATCGACAAAGCCGTGACAGTTCGAACGGACAAG
CTGGGGAAAGCTTTCGGTCAGCTGGACGATACCGCCATGATCGAGGTGAACCGGGCACTAGCACTGTTTTTAGGAGTGGC
GGGGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224680 WP_037931247.1 NZ_CP087054:30909-31127 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224680 NZ_CP087054:30909-31127 [Pseudosulfitobacter pseudonitzschiae]
ATGCCAACCTCTGTTTCTCACCGTGTCCAGAAGCGTCGCGATGCCTTGCGTGCCGCTGGTTTGCGCCCCGTCCAGATTTG
GGTGCCGGATACCCGCCGCCCCGGTTTCGAGGCTGAGTGCCGCCGCCAGGCTGCAATTGTAGCCAAGGCCGATCGCGCAG
ATGATACTCTGAACGATTTCCTGGATGCCTCCCTGGTCGATGTGGATGACTGGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4