Detailed information of TA system TA224679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 102769..103381
• Replicon: plasmid P2
• Accession: NZ_CP087053
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-43

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE36_RS20220
• Protein ID: WP_028288627.1
• Coordinates: 102986..103381 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE36_RS20215
• Protein ID: WP_009503866.1
• Coordinates: 102769..102996 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE36_RS20200 (LOE36_20215)	99026..100237	-	1212	WP_050525470.1	plasmid replication protein RepC	-
LOE36_RS20205 (LOE36_20220)	100451..101383	-	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE36_RS20210 (LOE36_20225)	101367..102560	-	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE36_RS20215 (LOE36_20230)	102769..102996	+	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE36_RS20220 (LOE36_20235)	102986..103381	+	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE36_RS20225 (LOE36_20240)	103460..103951	-	492	Protein_108	recombinase family protein	-
LOE36_RS20230 (LOE36_20245)	104053..105204	-	1152	WP_224557873.1	tyrosine-type recombinase/integrase	-
LOE36_RS20235 (LOE36_20250)	105319..106203	+	885	WP_037239259.1	DUF1403 family protein	-
LOE36_RS20240 (LOE36_20255)	106251..106862	+	612	WP_197919471.1	SMC-Scp complex subunit ScpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110566	110566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224679 WP_028288627.1 NZ_CP087053:102986-103381 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224679 NZ_CP120394:c4126327-4125839 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCGCCCCTGAACCGCTTCATGCCGGGCATATTCTTACCCCCTTCTGCTGCGGTGTGGATTCAATGGATAACTG
GCTAAAACAGCGGGCGATGAAAAATCAGGTAACTGGCGCATCCCGTACCTTTGTGTGTTGCGGCAGCGATTCTGACGTAC
TGGCGTATTATTCGCTGGCGTCCAGCGCGGTAACGACAAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTTGTGGTGCTGGGCCGTCTGGCGGTGGATCGGTCGCTACATGGGCAGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGCGGGATGCTGGTCCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224679 WP_009503866.1 NZ_CP087053:102769-102996 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224679 NZ_CP120394:c4126617-4126324 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGTCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGCTGCAAAAGCCAGAGGGAAAAACTGCACAGACTTTGTGCTAGAAGCTGCCCGCGCCGCCGCCGAGGAAGCGCTGA
TCGAGCAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGCATTTCTGATCCGTCTGGATCAGGCTCCTGCGCCAAAC
GATGCGTTGCGTAAAACCATGCAGACTCCTGCACCGTGGGAGCAGGAAAAATGA