Detailed information of TA system TA224673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 170756..171297
• Replicon: plasmid P2
• Accession: NZ_CP087044
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC52-18

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE42_RS21275
• Protein ID: WP_037931244.1
• Coordinates: 170971..171297 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE42_RS21270
• Protein ID: WP_037931247.1
• Coordinates: 170756..170974 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE42_RS21250 (LOE42_21245)	166013..167020	+	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-
LOE42_RS21255 (LOE42_21250)	167255..168802	+	1548	WP_073194592.1	AMP-binding protein	-
LOE42_RS21260 (LOE42_21255)	168857..169321	+	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE42_RS21265 (LOE42_21260)	169325..170458	+	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE42_RS21270 (LOE42_21265)	170756..170974	+	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE42_RS21275 (LOE42_21270)	170971..171297	+	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE42_RS21280 (LOE42_21275)	171471..171827	-	357	Protein_163	ATP-binding protein	-
LOE42_RS21285 (LOE42_21280)	171817..173389	-	1573	Protein_164	IS21 family transposase	-
LOE42_RS21290 (LOE42_21285)	173725..174048	-	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE42_RS21295 (LOE42_21290)	174211..174618	+	408	WP_157802081.1	DoxX family protein	-
LOE42_RS21300 (LOE42_21295)	174682..175497	+	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE42_RS21305 (LOE42_21300)	175506..176003	+	498	WP_037931962.1	DUF5519 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..193389	193389
	-	flank	IS/Tn	-	-	171817..173103	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224673 WP_037931244.1 NZ_CP087044:170971-171297 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224673 NZ_CP120394:820737-821135 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTGAAATTCATGCTTGATACCAATACTTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCACCGG
AGCGTAATTTTGCAGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTACCGAACTGGCCCGCAAGGGAACACCTGTCGGGTCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224673 WP_037931247.1 NZ_CP087044:170756-170974 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224673 NZ_CP120394:820510-820737 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGCACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4