Detailed information of TA system TA224666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 23501..24042
• Replicon: plasmid P3
• Accession: NZ_CP087035
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC52-67

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE43_RS20985
• Protein ID: WP_037931244.1
• Coordinates: 23501..23827 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE43_RS20990
• Protein ID: WP_037931247.1
• Coordinates: 23824..24042 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE43_RS20955 (LOE43_20955)	18795..19292	-	498	WP_037931962.1	DUF5519 family protein	-
LOE43_RS20960 (LOE43_20960)	19301..20116	-	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE43_RS20965 (LOE43_20965)	20180..20587	-	408	WP_157802081.1	DoxX family protein	-
LOE43_RS20970 (LOE43_20970)	20750..21073	+	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE43_RS20975 (LOE43_20975)	21409..22981	+	1573	Protein_25	IS21 family transposase	-
LOE43_RS20980 (LOE43_20980)	22971..23327	+	357	Protein_26	ATP-binding protein	-
LOE43_RS20985 (LOE43_20985)	23501..23827	-	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE43_RS20990 (LOE43_20990)	23824..24042	-	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE43_RS20995 (LOE43_20995)	24340..25473	-	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE43_RS21000 (LOE43_21000)	25477..25941	-	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE43_RS21005 (LOE43_21005)	25996..27543	-	1548	WP_073194592.1	AMP-binding protein	-
LOE43_RS21010 (LOE43_21010)	27778..28785	-	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..194794	194794
	-	flank	IS/Tn	-	-	21695..22981	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224666 WP_037931244.1 NZ_CP087035:c23827-23501 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224666 NZ_CP120393:c4006600-4006316 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224666 WP_037931247.1 NZ_CP087035:c24042-23824 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224666 NZ_CP120393:c4006832-4006590 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4