Detailed information of TA system TA224660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3315862..3316327
• Replicon: chromosome
• Accession: NZ_CP087025
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC52-68

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE44_RS16780
• Protein ID: WP_224557762.1
• Coordinates: 3315862..3316125 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE44_RS16785
• Protein ID: WP_224557764.1
• Coordinates: 3316112..3316327 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE44_RS16755 (LOE44_16745)	3311577..3313127	-	1551	WP_224557015.1	restriction endonuclease subunit S	-
LOE44_RS16760 (LOE44_16750)	3313124..3313420	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE44_RS16765 (LOE44_16755)	3313314..3313802	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE44_RS16770 (LOE44_16760)	3314028..3315138	-	1111	Protein_3314	IS110 family transposase	-
LOE44_RS16775 (LOE44_16765)	3315231..3315674	-	444	WP_224557760.1	hypothetical protein	-
LOE44_RS16780 (LOE44_16770)	3315862..3316125	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE44_RS16785 (LOE44_16775)	3316112..3316327	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE44_RS16790 (LOE44_16780)	3316680..3316904	-	225	WP_224557765.1	hypothetical protein	-
LOE44_RS16795 (LOE44_16785)	3317072..3317452	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE44_RS16800 (LOE44_16790)	3317633..3318604	-	972	WP_224557767.1	hypothetical protein	-
LOE44_RS16805 (LOE44_16795)	3318597..3319781	-	1185	WP_224557768.1	site-specific integrase	-
LOE44_RS16815 (LOE44_16805)	3320159..3320857	-	699	WP_037931342.1	SURF1 family protein	-
LOE44_RS16820 (LOE44_16810)	3320854..3321219	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3307267..3319781	12514

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224660 WP_224557762.1 NZ_CP087025:3315862-3316125 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224660 NZ_CP120393:805693-806094 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCCTTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGACAG
GACTGGACGCTCTTGCGTCCTCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
AG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224660 WP_224557764.1 NZ_CP087025:3316112-3316327 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224660 NZ_CP120393:805446-805712 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA