Detailed information of TA system TA224654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3203400..3203865
• Replicon: chromosome
• Accession: NZ_CP087016
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC52-70

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE45_RS16115
• Protein ID: WP_224557762.1
• Coordinates: 3203602..3203865 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE45_RS16110
• Protein ID: WP_224557764.1
• Coordinates: 3203400..3203615 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE45_RS16075 (LOE45_16090)	3198508..3198873	+	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-
LOE45_RS16080 (LOE45_16095)	3198870..3199568	+	699	WP_037931342.1	SURF1 family protein	-
LOE45_RS16090 (LOE45_16105)	3199946..3201130	+	1185	WP_224557768.1	site-specific integrase	-
LOE45_RS16095 (LOE45_16110)	3201123..3202094	+	972	WP_224557767.1	hypothetical protein	-
LOE45_RS16100 (LOE45_16115)	3202275..3202655	+	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE45_RS16105 (LOE45_16120)	3202823..3203047	+	225	WP_224557765.1	hypothetical protein	-
LOE45_RS16110 (LOE45_16125)	3203400..3203615	-	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE45_RS16115 (LOE45_16130)	3203602..3203865	-	264	WP_224557762.1	BrnT family toxin	Toxin
LOE45_RS16120 (LOE45_16135)	3204053..3204496	+	444	WP_224557760.1	hypothetical protein	-
LOE45_RS16125 (LOE45_16140)	3204589..3205698	+	1110	WP_224558030.1	IS110 family transposase	-
LOE45_RS16130 (LOE45_16145)	3205924..3206412	+	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE45_RS16135 (LOE45_16150)	3206336..3206602	+	267	WP_229938971.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE45_RS16140 (LOE45_16155)	3206599..3208149	+	1551	WP_224557015.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3201300..3212459	11159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224654 WP_224557762.1 NZ_CP087016:c3203865-3203602 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224654 NZ_CP120392:c4008872-4008588 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224654 WP_224557764.1 NZ_CP087016:c3203615-3203400 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224654 NZ_CP120392:c4009104-4008862 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA