Detailed information of TA system TA224650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 62224..62836
• Replicon: plasmid P2
• Accession: NZ_CP087011
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC52-71

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE46_RS20375
• Protein ID: WP_028288627.1
• Coordinates: 62224..62619 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A921TG38
• Locus tag: LOE46_RS20380
• Protein ID: WP_009503866.1
• Coordinates: 62609..62836 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE46_RS20355 (LOE46_20360)	58743..59354	-	612	WP_197919471.1	SMC-Scp complex subunit ScpB	-
LOE46_RS20360 (LOE46_20365)	59402..60286	-	885	WP_037239259.1	DUF1403 family protein	-
LOE46_RS20365 (LOE46_20370)	60413..61552	+	1140	WP_229940649.1	tyrosine-type recombinase/integrase	-
LOE46_RS20370 (LOE46_20375)	61654..62145	+	492	Protein_62	recombinase family protein	-
LOE46_RS20375 (LOE46_20380)	62224..62619	-	396	WP_028288627.1	PIN domain-containing protein	Toxin
LOE46_RS20380 (LOE46_20385)	62609..62836	-	228	WP_009503866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LOE46_RS20385 (LOE46_20390)	63045..64238	+	1194	WP_009503867.1	plasmid partitioning protein RepA	-
LOE46_RS20390 (LOE46_20395)	64222..65154	+	933	WP_028288626.1	plasmid partitioning protein RepB	-
LOE46_RS20395 (LOE46_20400)	65368..66579	+	1212	WP_050525470.1	plasmid replication protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110567	110567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-47)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14212.23 Da
• Isoelectric Point: 4.1653

>T224650 WP_028288627.1 NZ_CP087011:c62619-62224 [Pseudosulfitobacter pseudonitzschiae]
MSVEFADTNVVLYLLDDGPKADRAEVILGQGPRISVQVLNESLVNCRRKAGLGWEEAAAFLEGVRALCPVEDLTVQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T224650 NZ_CP120392:c974567-974040 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.79 Da
• Isoelectric Point: 11.0482

>AT224650 WP_009503866.1 NZ_CP087011:c62836-62609 [Pseudosulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGRVRVRRLARADEVLTGLRRFRGKLSAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT224650 NZ_CP120392:c974854-974564 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGATTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA