Detailed information of TA system TA224646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 29429..29990
• Replicon: plasmid P6
• Accession: NZ_CP087008
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-105

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOE06_RS23485
• Protein ID: WP_224557555.1
• Coordinates: 29715..29990 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LOE06_RS23480
• Protein ID: WP_224557554.1
• Coordinates: 29429..29722 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE06_RS23465 (LOE06_23440)	24906..26441	-	1536	WP_224557548.1	aldehyde dehydrogenase (NADP(+))	-
LOE06_RS23470 (LOE06_23445)	26703..28442	+	1740	WP_224557550.1	L-arabinonate dehydratase	-
LOE06_RS23475 (LOE06_23450)	28453..29358	+	906	WP_224557552.1	dihydrodipicolinate synthase family protein	-
LOE06_RS23480 (LOE06_23455)	29429..29722	+	294	WP_224557554.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LOE06_RS23485 (LOE06_23460)	29715..29990	+	276	WP_224557555.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOE06_RS23490 (LOE06_23465)	30459..31196	-	738	WP_229940771.1	GntR family transcriptional regulator	-
LOE06_RS23495 (LOE06_23470)	31321..32868	+	1548	WP_224557559.1	altronate dehydratase family protein	-
LOE06_RS23500 (LOE06_23475)	32885..34201	+	1317	WP_224557560.1	lactate racemase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82248	82248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-55)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10108.67 Da
• Isoelectric Point: 4.4912

>T224646 WP_224557555.1 NZ_CP087008:29715-29990 [Pseudosulfitobacter pseudonitzschiae]
VPDLEWKALAVADLMAIVDYISDDNPDAAISLMDDIQDKVLGLLTHPKSGRPGRVNDTRELVIRPNYIVVYAENTAAVTI
LRVLHAVQMWP

Nucleotide

• Length: 276 bp

>T224646 NZ_CP120377:c1009988-1009686 [Pseudomonas sp. G11]
ATGATTCAGTTTAAGAAGTCAAAACACTTTCTAGAATGGCTTGATTCGTTACGCAGTAAACCCGCGCGAGCACGCGTACT
GGCAAGGTTGGATCACGCGCAATGGGGCAACTTTGGAGACTGCGAAGCACTCGGAAATGGCGTTTTTGAAATGCGCATCC
ACTATGGCCCTGGCTACAGGGTTTATTTCACTCGCAGAGACGAAGTGGTTTACCTGCTGTTGATCGGAGGCGACAAGTCA
ACGCAGAAACGTGACATTCAGCGCGCTAAACAAATTGCAGAAGACTTTGAAAAAAAGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10166.59 Da
• Isoelectric Point: 7.2024

>AT224646 WP_224557554.1 NZ_CP087008:29429-29722 [Pseudosulfitobacter pseudonitzschiae]
MSAQTSMIHVRVDDQLKAKATDALSGVGLTLSDAVRILLTRVAAEGGLPAGLTANPDAYDAWFRAKVQDALADAAPAKPH
DQVMRDARALIDGKRGA

Nucleotide

• Length: 294 bp

>AT224646 NZ_CP120377:c1009682-1009395 [Pseudomonas sp. G11]
ATGAATGAATTCACTGACTTCGACGTGGCGGAGCACCTAAAAAACCCCGAGGATATGGCCGAATATCTTGAAGCGTGCTT
CGAAGAAGATACGGGTGACGGTCTGCTGATCCGTTCAGCGCTAAACAACATCGCCCGCGCCCAAGGCATGACCCAAGTAG
CCCGCGATGCCGGCCTCGGAAGAGAAAGTCTATACAAAGCCCTCTCCAGCAATGGCAATCCCGAGTTCGCGACTATCATG
AAAGTCATGAAGGCTTTGGGCCTCAAGCTTCACGCTACAGCGATCTGA