Detailed information of TA system TA224645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 76048..76722
• Replicon: plasmid P4
• Accession: NZ_CP087006
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-105

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE06_RS22505
• Protein ID: WP_224557924.1
• Coordinates: 76300..76722 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE06_RS22500
• Protein ID: WP_229939240.1
• Coordinates: 76048..76296 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE06_RS22480 (LOE06_22455)	71961..72605	+	645	WP_224557929.1	type IV secretion system protein	-
LOE06_RS22485 (LOE06_22460)	72609..73340	+	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE06_RS22490 (LOE06_22465)	73343..74800	+	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE06_RS22495 (LOE06_22470)	74911..75900	+	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE06_RS22500 (LOE06_22475)	76048..76296	+	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE06_RS22505 (LOE06_22480)	76300..76722	+	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE06_RS22510 (LOE06_22485)	76719..79469	+	2751	WP_224557923.1	lactate dehydrogenase	-
LOE06_RS22515 (LOE06_22490)	79466..80524	+	1059	WP_224557922.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..157667	157667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224645 WP_224557924.1 NZ_CP087006:76300-76722 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224645 NZ_CP120377:c1009682-1009395 [Pseudomonas sp. G11]
ATGAATGAATTCACTGACTTCGACGTGGCGGAGCACCTAAAAAACCCCGAGGATATGGCCGAATATCTTGAAGCGTGCTT
CGAAGAAGATACGGGTGACGGTCTGCTGATCCGTTCAGCGCTAAACAACATCGCCCGCGCCCAAGGCATGACCCAAGTAG
CCCGCGATGCCGGCCTCGGAAGAGAAAGTCTATACAAAGCCCTCTCCAGCAATGGCAATCCCGAGTTCGCGACTATCATG
AAAGTCATGAAGGCTTTGGGCCTCAAGCTTCACGCTACAGCGATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224645 WP_229939240.1 NZ_CP087006:76048-76296 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224645 NZ_CP120377:c1009988-1009686 [Pseudomonas sp. G11]
ATGATTCAGTTTAAGAAGTCAAAACACTTTCTAGAATGGCTTGATTCGTTACGCAGTAAACCCGCGCGAGCACGCGTACT
GGCAAGGTTGGATCACGCGCAATGGGGCAACTTTGGAGACTGCGAAGCACTCGGAAATGGCGTTTTTGAAATGCGCATCC
ACTATGGCCCTGGCTACAGGGTTTATTTCACTCGCAGAGACGAAGTGGTTTACCTGCTGTTGATCGGAGGCGACAAGTCA
ACGCAGAAACGTGACATTCAGCGCGCTAAACAAATTGCAGAAGACTTTGAAAAAAAGGAGTAA