22464

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 3425735..3427070 Replicon chromosome
Accession NC_009801
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name sanaT Uniprot ID A7ZRI5
Locus tag ECE24377A_RS18715 Protein ID WP_012139193.1
Coordinates 3426123..3427070 (+) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID A0A0Q1C5A3
Locus tag ECE24377A_RS18710 Protein ID WP_000208385.1
Coordinates 3425735..3426130 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS18665 3420932..3421750 + 819 WP_001234690.1 DUF945 domain-containing protein -
ECE24377A_RS18670 3421842..3422327 + 486 WP_000213725.1 antirestriction protein -
ECE24377A_RS18675 3422343..3422819 + 477 WP_001186781.1 RadC family protein -
ECE24377A_RS18680 3422906..3423127 + 222 WP_000692348.1 DUF987 domain-containing protein -
ECE24377A_RS18685 3423207..3423575 + 369 WP_012139188.1 type IV toxin-antitoxin system toxin CbtA -
ECE24377A_RS18690 3423665..3424042 + 378 WP_000854738.1 TA system toxin CbtA family protein -
ECE24377A_RS18695 3424039..3424527 + 489 WP_000761672.1 hypothetical protein -
ECE24377A_RS18700 3424539..3424736 + 198 WP_000839234.1 DUF957 domain-containing protein -
ECE24377A_RS18705 3424821..3425666 + 846 WP_000065749.1 DUF4942 domain-containing protein -
ECE24377A_RS18710 3425735..3426130 + 396 WP_000208385.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
ECE24377A_RS18715 3426123..3427070 + 948 WP_012139193.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
ECE24377A_RS18720 3427425..3427961 - 537 WP_000942795.1 GspM family type II secretion system protein YghD -
ECE24377A_RS18725 3427963..3429141 - 1179 WP_000094974.1 type II secretion system protein GspL -
ECE24377A_RS18730 3429138..3430115 - 978 WP_000633187.1 type II secretion system minor pseudopilin GspK -
ECE24377A_RS18735 3430118..3430717 - 600 WP_001255040.1 type II secretion system minor pseudopilin GspJ -
ECE24377A_RS18740 3430714..3431085 - 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
ECE24377A_RS18745 3431082..3431645 - 564 WP_001115154.1 type II secretion system minor pseudopilin GspH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC / rfaE 3348871..3530406 181535


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(36-302)

Antitoxin

Protein of unknown function DUF6088

(10-129)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 36147.49 Da        Isoelectric Point: 5.7165

>T22464 WP_012139193.1 NC_009801:3426123-3427070 [Escherichia coli O139:H28 str. E24377A]
MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETHTLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDKRRKIRKEIVQIITDVLHNSDIFSLDNENARITRDEYRYNEIPVRYPQAFAQAPCLRPFIKLELMETTLLEHPESR
DIYSLVTEVTGNGTPVTAFPCATILSTQAEKLISMMRRTAILLRNPEQQDDESLVRHIYDNYCIVREKGVNVPVLKNFVQ
VCIQQDIERYGNQHPQFCNSPVDELKKGLDALRDDPVHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTS

Download         Length: 948 bp

>T22464 NC_009801:3426123-3427070 [Escherichia coli O139:H28 str. E24377A]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCAACGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTAATCTTTTAAAGTTACTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGAGGTACCGCAT
TATCAAAAGCAGGTATCTCATTGAACAGAATGTCAGAAGATATTGACATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTCTTCATAATTCTGACATTTT
CAGCCTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAGATCCCCGTACGTTATCCCCAGGCAT
TTGCCCAGGCCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAACCACTCTGCTGGAACATCCGGAAAGCAGA
GATATTTACTCACTTGTCACGGAGGTAACGGGTAATGGCACGCCTGTAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATATCCATGATGCGAAGAACAGCCATACTTCTTCGCAATCCCGAACAACAGGATGATGAGTCCC
TGGTACGCCACATTTACGACAATTATTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCAGTACTGAAAAATTTTGTTCAG
GTATGCATTCAACAGGATATTGAGCGCTATGGTAACCAGCACCCACAATTTTGTAACTCACCTGTTGATGAACTTAAAAA
AGGGCTGGATGCACTCCGGGATGATCCTGTACACAAATTGCGTTTTCAACGATTTGTTAAACCGATGGTATTTGGTAATA
CACAGATTGACTGGGAAGAAGCCTACTCCTGTTTCAGACAAACAGCATTGAGTGTTCTGACCAGCTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14595.97 Da        Isoelectric Point: 10.9440

>AT22464 WP_000208385.1 NC_009801:3425735-3426130 [Escherichia coli O139:H28 str. E24377A]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITE
ALDLLNIRYSLIGATECYNKGQSTQIPVSPEFKTPPRFKRVLVVGKSKINA

Download         Length: 396 bp

>AT22464 NC_009801:3425735-3426130 [Escherichia coli O139:H28 str. E24377A]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAGCGTTATGTGTTCACCCGGGATGACTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAGGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGCAACGTTATTACAGGAAAAATAATGCCTGCAGCACCTGGTGGCTCTGCTGCTGTAATCACCGAA
GCCCTGGATCTTTTGAATATCAGATACAGCTTGATCGGCGCAACAGAATGCTACAATAAAGGTCAGTCCACACAGATTCC
GGTGTCTCCGGAGTTCAAAACACCTCCTCGCTTTAAAAGAGTGTTAGTTGTAGGGAAAAGTAAAATCAATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZRI5

Antitoxin

Source ID Structure
AlphaFold DB A0A0Q1C5A3

References