Detailed information of TA system TA224638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 209460..210001
• Replicon: plasmid P2
• Accession: NZ_CP086997
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-109

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE07_RS21595
• Protein ID: WP_037931244.1
• Coordinates: 209675..210001 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE07_RS21590
• Protein ID: WP_037931247.1
• Coordinates: 209460..209678 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE07_RS21570 (LOE07_21545)	204717..205724	+	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-
LOE07_RS21575 (LOE07_21550)	205959..207506	+	1548	WP_073194592.1	AMP-binding protein	-
LOE07_RS21580 (LOE07_21555)	207561..208025	+	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE07_RS21585 (LOE07_21560)	208029..209162	+	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE07_RS21590 (LOE07_21565)	209460..209678	+	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE07_RS21595 (LOE07_21570)	209675..210001	+	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE07_RS21600 (LOE07_21575)	210175..210531	-	357	Protein_198	ATP-binding protein	-
LOE07_RS21605 (LOE07_21580)	210521..212093	-	1573	Protein_199	IS21 family transposase	-
LOE07_RS21610 (LOE07_21585)	212429..212752	-	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE07_RS21615 (LOE07_21590)	212915..213322	+	408	WP_157802081.1	DoxX family protein	-
LOE07_RS21620 (LOE07_21595)	213386..214201	+	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE07_RS21625 (LOE07_21600)	214210..214707	+	498	WP_037931962.1	DUF5519 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290593	290593
	-	flank	IS/Tn	-	-	210521..211807	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224638 WP_037931244.1 NZ_CP086997:209675-210001 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224638 NZ_CP120376:116695-116970 [Pseudomonas nitroreducens]
ATGCGAGTGATCTACGCGCCCGCGGCGCTTCGCGACCTCGACCGCCTGCGCCGATTCCTCCGGGAAACCAACCCCACCGC
CGCCCGCCGCGCCGGCCAGATCATTCTCCAGGCCACCCAGGCGCTGGGGGCCTATCCGCAGATGGGGCGACTGATCGATG
ACCTGCCGATCGAATTCCGCGAATGGCCCATCGACTTCGGTGACAGCGGCTACCTAGCTCGCTACCACATCGATGGAGAA
ACACTGGTCACCCTCGCGATCCGCCACCAGCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224638 WP_037931247.1 NZ_CP086997:209460-209678 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224638 NZ_CP120376:116435-116704 [Pseudomonas nitroreducens]
ATGGCCACTTCAGTCAAACTCGACGAGGAAATGCTCGCCCGCGTCCGCGAGCTGGCCGAACTGCGCCAGCGCTCGCCGCA
CTGGATCATGCGTGAAGCCATCGGCCAATACGTCGTGCGTGAAGAGCAGCGCGAGAAGCTCAAGCGCGACACCGAGCAGG
CCTGGGAAGAATTCCAGGCGACCGGCCAGCACGTCACTGCCGAAGCGGTGGAGAGCTGGCTGAACAGCTGGGGCGATGAG
GACGAGCAAGCCGCACCAAAATGCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4