Detailed information of TA system TA224637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 23856..24530
• Replicon: plasmid P2
• Accession: NZ_CP086997
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-109

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOE07_RS20695
• Protein ID: WP_224557924.1
• Coordinates: 23856..24278 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LOE07_RS20700
• Protein ID: WP_229939240.1
• Coordinates: 24282..24530 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE07_RS20685 (LOE07_20670)	20054..21112	-	1059	WP_224557922.1	HEPN domain-containing protein	-
LOE07_RS20690 (LOE07_20675)	21109..23859	-	2751	WP_224557923.1	lactate dehydrogenase	-
LOE07_RS20695 (LOE07_20680)	23856..24278	-	423	WP_224557924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOE07_RS20700 (LOE07_20685)	24282..24530	-	249	WP_229939240.1	plasmid stabilization protein	Antitoxin
LOE07_RS20705 (LOE07_20690)	24678..25667	-	990	WP_224557926.1	P-type DNA transfer ATPase VirB11	-
LOE07_RS20710 (LOE07_20695)	25778..27235	-	1458	WP_224557927.1	TrbI/VirB10 family protein	-
LOE07_RS20715 (LOE07_20700)	27238..27969	-	732	WP_224557928.1	TrbG/VirB9 family P-type conjugative transfer protein	-
LOE07_RS20720 (LOE07_20705)	27973..28617	-	645	WP_224557929.1	type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..290593	290593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15255.26 Da
• Isoelectric Point: 4.3767

>T224637 WP_224557924.1 NZ_CP086997:c24278-23856 [Pseudosulfitobacter pseudonitzschiae]
MIILDTNVICELLRPTPEPRIEQWLSAQDGLNVYLTSISEAELRYGLANMGNGKRRAALIDAVDRILREDLAGRILPFDS
DAAQAYASIAATRRDAGRPIAQADCQIASIAHACGATVATRNTPDFEGCEIDLINPWTTE

Nucleotide

• Length: 423 bp

>T224637 NZ_CP120374:c1765799-1765524 [Sinorhizobium garamanticum]
ATGAAGCTCCATTGGACGACCAAGTCGGTTTCTGACCTCGGACGCCTCTATGATTTTCTAGCACCGGTCAATCGACAAGC
AGAAGCCCGCACGGTGCAGGCACTGGCGAGCGCCCCAAGGCGCCTGCTCGAACAGCCACGGATCGGCGAACGGCTGGAAG
AGTTCGATCCCCGCGCGGTCCGTCGTATTCTCGTTAGCCATTACGAAATGCGCTATGAGATCACGCAGTCGACAATCTAC
GTGCTGAGGCTTTGGCACACGCGCGAGGATCGCTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9206.46 Da
• Isoelectric Point: 6.7281

>AT224637 WP_229939240.1 NZ_CP086997:c24530-24282 [Pseudosulfitobacter pseudonitzschiae]
MASITIRNLDDDVKRRLRIRAAEHGRSMEEEAREILRQVVGDAKPNHDLAAAIRARVGPLGGVELDLPQREAMHEPPTFD
RA

Nucleotide

• Length: 249 bp

>AT224637 NZ_CP120374:c1766059-1765799 [Sinorhizobium garamanticum]
ATGGAAACGAAGGTGCTGACAGCACATGTGCCGCTGCCGCTCGCAGAAAAGGTCGATCAGATTGCGGCACGGCTCGAACG
TTCGCGCGGCTGGATCGTCAAGCAGGCGCTGACCGCCTGGATCGATCAGGAAGAGGAGCGCCGGCGCTTGACGCTGGAGG
CGCTCGCCAATGTCGACGGGGGCCATGTCGTCAGCCACCAGTCCGTGCAAGCTTGGGCCGACAGCCTCGATAGTGACAAG
CCGCTGCCGCTGCCACTTTGA