Detailed information of TA system TA224633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 22094..22635
• Replicon: plasmid P8
• Accession: NZ_CP086994
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-110

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A073IW87
• Locus tag: LOE08_RS23850
• Protein ID: WP_037931244.1
• Coordinates: 22094..22420 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A073IWP4
• Locus tag: LOE08_RS23855
• Protein ID: WP_037931247.1
• Coordinates: 22417..22635 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE08_RS23820 (LOE08_23770)	17388..17885	-	498	WP_037931962.1	DUF5519 family protein	-
LOE08_RS23825 (LOE08_23775)	17894..18709	-	816	WP_224556226.1	class III extradiol ring-cleavage dioxygenase	-
LOE08_RS23830 (LOE08_23780)	18773..19180	-	408	WP_157802081.1	DoxX family protein	-
LOE08_RS23835 (LOE08_23785)	19343..19666	+	324	WP_027238707.1	helix-turn-helix domain-containing protein	-
LOE08_RS23840 (LOE08_23790)	20002..21574	+	1573	Protein_24	IS21 family transposase	-
LOE08_RS23845 (LOE08_23795)	21564..21920	+	357	Protein_25	ATP-binding protein	-
LOE08_RS23850 (LOE08_23800)	22094..22420	-	327	WP_037931244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOE08_RS23855 (LOE08_23805)	22417..22635	-	219	WP_037931247.1	antitoxin MazE family protein	Antitoxin
LOE08_RS23860 (LOE08_23810)	22933..24066	-	1134	WP_037931249.1	alpha/beta fold hydrolase	-
LOE08_RS23865 (LOE08_23815)	24070..24534	-	465	WP_051694690.1	MaoC/PaaZ C-terminal domain-containing protein	-
LOE08_RS23870 (LOE08_23820)	24589..26136	-	1548	WP_073194592.1	AMP-binding protein	-
LOE08_RS23875 (LOE08_23825)	26371..27378	-	1008	WP_081855484.1	PaaX family transcriptional regulator C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..193372	193372
	-	flank	IS/Tn	-	-	20288..21574	1286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11610.50 Da
• Isoelectric Point: 8.8545

>T224633 WP_037931244.1 NZ_CP086994:c22420-22094 [Pseudosulfitobacter pseudonitzschiae]
VRRGELVTVALQGDHGKPRPALVIQSDRFPDTATTTVLLVTSTLVDAPLIRLTVEPSAENGLRMRSQIMIDKAVTVRTDK
LGKAFGQLDDTAMIEVNRALALFLGVAG

Nucleotide

• Length: 327 bp

>T224633 NZ_CP120372:c163596-163189 [Sinorhizobium numidicum]
ATGTATCTGGTTGACACCAACATCGTCTCGGAGGCGCGACGCGGCGCGCCGCAGGCGGTTGCCTGGCTGCGCTCGGTCGA
CCCGCTCAGCATCCATCTGAGCGCGCTGACCCTCGGCGAAATCATGCGCGGCATTGCGCTGAAACAGAAGTCGGATCCGA
AGGCCGCCGCTCACCTCGCCGAATGGCTGCGCAAGCTGCGCCACGACCACGGCGACCGTATCCTGCCGGTGACCGACCAG
ATCGCCGTCGAATGGGGCCGCATCGCCGCGATCCGACCGCGTGGCGATATTGACGGGCTCCTCGCCGCGACTGCGGTCGT
CCACGACCTGATCCTCGTCACGCGCAACGTCAAGGATTTTGAAGACACGGACGCTTCCGTGATCAATCCGTGGGAGACAC
CGGCGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8175.17 Da
• Isoelectric Point: 5.8017

>AT224633 WP_037931247.1 NZ_CP086994:c22635-22417 [Pseudosulfitobacter pseudonitzschiae]
MPTSVSHRVQKRRDALRAAGLRPVQIWVPDTRRPGFEAECRRQAAIVAKADRADDTLNDFLDASLVDVDDWE

Nucleotide

• Length: 219 bp

>AT224633 NZ_CP120372:c163850-163596 [Sinorhizobium numidicum]
ATGGAATGGCAGCTGCAGGACGCCAAGAACCACTTCTCGAAAGTGGTGCAGAAGGCGCGACACGAGGGGCCGCAGATCGT
CACGCTGCGTGGCGAGCGCACGGCCGTAGTGCTGTCCGCCCACGACTATGACGCTCTGCGCGCCGGCCGACCGACGCTGG
TCGACGATCTGCTCGGCGGTCCCCCTTGGGACGATGAGCTCGCCGATGCGGTCGAGGCGCGCGCCAAGACGCCAAGCCGC
GACGTGGCCTTCTAA

Structures

Toxin

• AlphaFold DB: A0A073IW87

Antitoxin

• AlphaFold DB: A0A073IWP4