Detailed information of TA system TA224629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 643570..644035
• Replicon: chromosome
• Accession: NZ_CP086986
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-110

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE08_RS03090
• Protein ID: WP_224557762.1
• Coordinates: 643570..643833 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE08_RS03095
• Protein ID: WP_224557764.1
• Coordinates: 643820..644035 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE08_RS03070 (LOE08_03080)	640833..641129	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE08_RS03075 (LOE08_03085)	641023..641511	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE08_RS03080 (LOE08_03090)	641737..642846	-	1110	WP_224558030.1	IS110 family transposase	-
LOE08_RS03085 (LOE08_03095)	642939..643382	-	444	WP_224557760.1	hypothetical protein	-
LOE08_RS03090 (LOE08_03100)	643570..643833	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE08_RS03095 (LOE08_03105)	643820..644035	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE08_RS03100 (LOE08_03110)	644388..644612	-	225	WP_224557765.1	hypothetical protein	-
LOE08_RS03105 (LOE08_03115)	644780..645160	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE08_RS03110 (LOE08_03120)	645341..646312	-	972	WP_224557767.1	hypothetical protein	-
LOE08_RS03115 (LOE08_03125)	646305..647489	-	1185	WP_224557768.1	site-specific integrase	-
LOE08_RS03125 (LOE08_03135)	647867..648565	-	699	WP_037931342.1	SURF1 family protein	-
LOE08_RS03130 (LOE08_03140)	648562..648927	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	636237..647489	11252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224629 WP_224557762.1 NZ_CP086986:643570-643833 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224629 NZ_CP120370:c301638-301255 [Sinorhizobium numidicum]
GTGAGGCTGGCCGACACGGGAATCTGGATCGACCACTTCCGCCTAGCTGATCCGCAGCTAATCCAAACGATCGAAAACGA
TATGCTGCTCTGCCATCCCGCCATCGTTGGCGAACTTGCGTTGGGCAGCTTGCACAATCGTCTGGTCACGCTCGGGTTCT
TGGCGGTCCAACGGGAGGTCATCATCGCCACGCACGATGAAGTCATGGCAATGATCGACGAGCACCAACTTTACAGCATG
GGCATAGGCTACACGGACGCCCATCTGCTCGCGTCAACTTTAATCGACAGCCGAGCCGAGTTGTGGACGCGAGACAAGCG
GCTGCGCAGGGCGGCAGAAAAAGCGCGCGCGCGGGTGGTTTCTGACGGTACTGTGCCCAACTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224629 WP_224557764.1 NZ_CP086986:643820-644035 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224629 NZ_CP120370:c301829-301635 [Sinorhizobium numidicum]
ATGCGGTCAACCATCAACATAGACGACAGCCTCATGGAAAAGGCGAAGGCCCTGACCGGCACGAAAGAAACGGCGGCGGT
CGTCCGTCAGGCGCTGGAAACGCTGGTTCGCATGGAAGCAGGCAAGCGTCTGATCGCCTTGGGCGGCACGATGCCCGACG
CGGAGGCTGGTCCGCGGAGGCGGGCCGAAAAGTGA