Detailed information of TA system TA224624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3811755..3812220
• Replicon: chromosome
• Accession: NZ_CP086979
• Organism: Pseudosulfitobacter pseudonitzschiae strain SC1-111

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE09_RS19075
• Protein ID: WP_224557762.1
• Coordinates: 3811755..3812018 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LOE09_RS19080
• Protein ID: WP_224557764.1
• Coordinates: 3812005..3812220 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE09_RS19050 (LOE09_19045)	3807471..3809021	-	1551	WP_224557015.1	restriction endonuclease subunit S	-
LOE09_RS19055 (LOE09_19050)	3809018..3809314	-	297	WP_224557153.1	type I restriction-modification enzyme R subunit C-terminal domain-containing protein	-
LOE09_RS19060 (LOE09_19055)	3809208..3809696	-	489	WP_229938596.1	DEAD/DEAH box helicase family protein	-
LOE09_RS19065 (LOE09_19060)	3809922..3811031	-	1110	WP_224558030.1	IS110 family transposase	-
LOE09_RS19070 (LOE09_19065)	3811124..3811567	-	444	WP_224557760.1	hypothetical protein	-
LOE09_RS19075 (LOE09_19070)	3811755..3812018	+	264	WP_224557762.1	BrnT family toxin	Toxin
LOE09_RS19080 (LOE09_19075)	3812005..3812220	+	216	WP_224557764.1	CopG family antitoxin	Antitoxin
LOE09_RS19085 (LOE09_19080)	3812573..3812797	-	225	WP_224557765.1	hypothetical protein	-
LOE09_RS19090 (LOE09_19085)	3812965..3813345	-	381	WP_224557766.1	helix-turn-helix domain-containing protein	-
LOE09_RS19095 (LOE09_19090)	3813526..3814497	-	972	WP_224557767.1	hypothetical protein	-
LOE09_RS19100 (LOE09_19095)	3814490..3815674	-	1185	WP_224557768.1	site-specific integrase	-
LOE09_RS19110 (LOE09_19105)	3816052..3816750	-	699	WP_037931342.1	SURF1 family protein	-
LOE09_RS19115 (LOE09_19110)	3816747..3817112	-	366	WP_028957811.1	cytochrome o ubiquinol oxidase subunit IV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3803161..3815674	12513

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.52 Da
• Isoelectric Point: 5.6550

>T224624 WP_224557762.1 NZ_CP086979:3811755-3812018 [Pseudosulfitobacter pseudonitzschiae]
MKFEYDPDKSAGNKAKHGIDFDEAQALWEDPWLLEAPARTDDEPRFFAIGMIKERHWSAVCTYRGDIIRIISVRRARKQE
IEHYEST

Nucleotide

• Length: 264 bp

>T224624 NZ_CP120367:c585186-584749 [Sinorhizobium numidicum]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATCGCCTTAATCGATCCGGGCCATGTAGCCCATGATGACGCGCACGATTG
GTTCGCATCGATCGGCCAGACCGCGTGGGCTACCTGCCCCATTACTGAAAATGGTGTCATCCGGATCGTGGGCAACGCCA
AGTATCCCAACTCTCCCGGCTCACCGTCACTCGTGATGGAGATCGTTGGCAAATTGCGGTCGCTACCCGGTCACTGTTTT
TGGCCGGACGATGTGAGCCTCGTCGGGTCAGGTGATATCGCTGCCACGAAAATCCTAACTTCGGGGCAGGTAACGGACAC
CTATCTGCTTGCACTCGCGAAGGCGCATGGGGGCCAGTTGGCGACATTCGATCGCAAGCTGTCAGCAGCGGCGGTCACAA
GGGGCAATGCTGCCTTGCGCCTGATCACCAGCAAATAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8265.40 Da
• Isoelectric Point: 10.6538

>AT224624 WP_224557764.1 NZ_CP086979:3812005-3812220 [Pseudosulfitobacter pseudonitzschiae]
MKAREFDQKFDTGENVSDLVDWSKARRTNTQTKRVNVDFPSWVVSGLDQKAKQLGVTRQSLIKLWIAERLQ

Nucleotide

• Length: 216 bp

>AT224624 NZ_CP120367:c585410-585183 [Sinorhizobium numidicum]
ATGAGAACGACACTGGCGATCGATGACGATGTCCTGATCGCCGCCAAAGCGATGGCAGCCCAGCAGCACCGTAGTGTCGG
CGAGGTCATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCTCGCAGCGGCGGCGAGCGCAATGGAATTCCTCTTCTGT
CACCTCGGCCGGACGCGCCGCCAGTGACTCTCGAGATCGTCAACGCCTTGCGTGACGAACTGCCGTGA